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| Ribeiro,Carlos Alexandre Gomes; Tanure,Janaína Paula Marques; Maciel,Talles Eduardo Ferreira; Barros,Everaldo Gonçalves de. |
| The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Cultivar protection; Genetic diversity; Genotyping method; Random identity probability. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300005 |
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| Ribeiro,Carlos Alexandre Gomes; Pinto,Marcos de Oliveira; Maciel,Talles Eduardo Ferreira; Pastina,Maria Marta; Barros,Everaldo Gonçalves de; Guimarães,Claudia Teixeira. |
| ABSTRACT The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Fingerprinting; Genotyping; Cultivar protection. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200163 |
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