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| Registros recuperados: 33 | |
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| Alvarado Castillo, Gerardo. |
| Morchella esculenta es un hongo comestible de alto valor comercial y la obtención de esclerocios es considerada como la clave para su cultivo. En este trabajo se evaluó la cepa IE-750 en ocho medios de cultivo sólido y seis en forma líquida, utilizando como parámetros el crecimiento micelial, la producción de biomasa, la habilidad para producir esclerocios y su biomasa. El mejor crecimiento micelial se obtuvo en el tratamiento con composta (T7: 53.87 cm2), y en cuanto a la biomasa, el medio de cultivo con levadura fue mejor (T3: 80.3 mg). Los tratamientos con gallinaza y composta, presentaron 40% y 80% de esclerocios, respectivamente, tanto en medio sólido como líquido. La mayor cantidad de biomasa de estas estructuras se presentó en el... |
| Tipo: Tesis |
Palavras-chave: Esclerocios; Composta; Medios de cultivo; Morchella esculenta; Maestría; Agroecosistemas Tropicales; Sclerotia; Compost; Culture media. |
| Ano: 2008 |
URL: http://hdl.handle.net/10521/1414 |
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| Kightlinger,Weston; Chen,Kai; Pourmir,Azadeh; Crunkleton,Daniel W; Price,Geoffrey L; Johannes,Tyler W. |
| Background: Algae offer many advantages as biofuel sources including: high growth rates, high lipid content, the ability to grow on non-agricultural land, and the genetic versatility to improve strains rapidly and produce co-products. Research is ongoing to make algae biofuels a more financially attractive energy option; however, it is becoming evident that the economic viability of algae-based fuels may hinge upon high-value co-products. This work evaluated the feasibility of using a co-product, algae extract, as a nutrient source in cell culture media. Results: Algae extract prepared from autolysed Chlamydomonas reinhardtii was found to contain 3.0% protein, 9.2% total carbohydrate, and 3.9% free α-amino acid which is similar to the nutrient... |
| Tipo: Journal article |
Palavras-chave: Algae extract Algae; Chlamydomonas reinhardtii; Microalgae; Culture media. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100003 |
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| PEREIRA,GUILLERMO; ALBORNOZ,VERÓNICA; ROMERO,CHRISTIAN; LARA,SEBASTIÁN; SÁNCHEZ-OLATE,MANUEL; RÍOS,DARCY; ATALA,CRISTIAN. |
| ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C. gavilu and C. virescens were manually autopollinated. The resulting capsules were sterilized and seeds were aseptically obtained. We evaluated asymbiotic germination in: Agar Water (AW), Knudson C (KC), Banana Culture Media (CMB), Tomato Culture Media (CMT), Malmgren Modified (MM), Murashige and Skoog (MS), and MS modified (MS1/2). Seeds were incubated in the dark at 24±1 ºC for 2 weeks. Then they were put in 16/8 h light/dark cycles for 14 weeks. We... |
| Tipo: Journal article |
Palavras-chave: Terrestrial orchids; Endemic species; In vitro germination; Culture media; Orchid propagation; Asymbiotic germination. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100131 |
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| White, D. |
| Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from... |
| Tipo: Text |
Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf |
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| Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R. |
| A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene. |
| Tipo: Text |
Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf |
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| Morais-Lino,Lucymeire Souza; Santos-Serejo,Janay Almeida dos; Silva,Sebastião de Oliveira e; Santana,José Raniere Ferreira de; Kobayashi,Adilson Kenji. |
| The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000010 |
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| Araruna,Elequisandra da Costa; Ribeiro-Oliveira,João Paulo; Pereira,Vanderley José; Asmar,Simone Abreu; Melo,Berildo de. |
| Abstract: The objective of this work was to evaluate how salt concentrations in media used for in vitro cultures affect the early development of “barueiro” (Dipteryx alata) seedlings obtained from the shoot apex. Two different in vitro culture media - Murashige & Skoog (MS) and wood plant medium (WPM) - in different salt concentrations (25, 50, 75, and 100%) were used. At 120 days, the D. alata seedlings in the MS medium, in its original concentration (100%), developed better than in other concentrations or in the WPM. Therefore, the MS medium was considered the most suitable for ensuring in vitro establishment because it provided greater root length (27.65 cm) and number of leaves per plant (26 leaves) than the other medium. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado biome; Culture media; Osmoregulation; Plant growth; Salt concentrations; Tissue culture. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201295 |
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| Registros recuperados: 33 | |
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