|
|
|
| Registros recuperados: 27 | |
|
| |
|
|
| Brondani, Gilvano Ebling; Universidade Federal do Paraná; Dutra, Leonardo Ferreira; Empresa Brasileira de Pesquisa Agropecuária; Wendling, Ivar; Empresa Brasileira de Pesquisa Agropecuária; Grossi, Fernando; Universidade Federal do Paraná; Hansel, Fabricio Augusto; Empresa Brasileira de Pesquisa Agropecuária; Araujo, Marla Alessandra; Universidade Federal de Lavras. |
| This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1) for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1) and GA3 (0, 0.1, 0.2 and 0.3 mg L-1). The 0.50 mg L-1 BAP and 0.050 mg... |
| Tipo: Pesquisa |
Palavras-chave: 5.01.03.04-0 in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3 Produção de Mudas in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/8317 |
| |
|
|
| Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Rodrigues, Filipe Almendagna; Universidade Federal de Lavras; Villa, Fabiola; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras. |
| O objetivo deste trabalho foi determinar a fonte de silício que proporcione melhor crescimento em plântulas de Cattleya loddigesii. Plântulas oriundas de sementes germinadas in vitro com aproximadamente 1,0 cm de comprimento foram inoculadas em tubos de ensaio contendo 15 mL de meio de cultura Knudson C modificado, acrescido de silicato de sódio (0, 5, 10 e 20 mg L-1) e silicato de potássio (0, 5, 10 e 20 mL L-1), em todas as combinações possíveis. O meio de cultura teve seu pH ajustado para 5,8 ± 0,1 e solidificado com 5 g L-1 de ágar antes do processo de autoclavagem a 121°C e 1 atm por 20 min. O cultivo foi mantido em sala de crescimento com irradiância de 35 µmol m-2 s-1, temperatura de 25 ± 1°C e... |
|
Palavras-chave: 5.01.03.00-8 Cattleya loddigesii; Meio de cultura; Orchidaceae; Cultura de tecidos Fitotecnia/Biotecnologia Cattleya loddigesii; Culture medium; Orchidaceae; Tissue culture. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6281 |
| |
|
|
| Martendal, Cíntia de Oliveira; Universidade Estadual de Maringá; Bernardino, Murilo Martins; Instituto Federal Goiano; Pereira, Flávia Dionísio; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.; Silva, Fabiano Guimarães; Instituto Federal Goiano; de Menezes, Carlos César Evangelista; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.; Monteiro Hara, Alessandra Cristina Boffino de Almeida; Instituto Federal Goiano. |
| The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss.) using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5%) with or without 70% alcohol was used to avoid... |
| Tipo: Pesquisa Empírica de Campo |
Palavras-chave: 5.01.03.04-0 tissue culture; Asepsis; Culture medium; Cerrado fruit Fitotecnia. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15402 |
| |
|
|
| Zanandrea,Ilisandra; Bacarin,Marcos Antonio; Braga,Eugenia Jacira Bolacel; Bianchi,Valmor João; Peters,José Antonio. |
| The aim of the present study was to evaluate the growth and development of apple rootstock shoots submitted to different photon flux densities (7, 14, 21 and 60 µmol m-2 s-1) and three culture media. The best photon flux for maximizing growth, number of shoots and leaves was 14 µmol m-2 s-1, which also resulted in the highest values of chloroplastic pigments. On the other hand, the highest photon flux (60 µmol m-2 s-1) decreased significantly these parameters. Medium with reduced sucrose concentration did not affect the analyzed parameters, enabling an efficient multiplication of the cultivar with only 1% of sucrose. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mallus; Apple; Sucrose; Photon flux density; Culture medium. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500005 |
| |
|
|
| Chen,Yong-Hao; Li,Jun; Liu,Li; Liu,Hong-Zhi; Wang,Qiang. |
| A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus equisimilis mutant; Hyaluronic acid; Optimization; Culture medium; Culture conditions. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400040 |
| |
|
|
| Paz,Marcelo Fossa da; Baruque-Ramos,Júlia; Hiss,Haroldo; Vicentin,Márcio Alberto; Leal,Maria Betania Batista; Raw,Isaías. |
| Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120 rpm and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neisseria meningitidis; Culture medium; Vaccine; Polysaccharide; Batch cultivation. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100007 |
| |
|
|
| Silva,Carlos Henrique Pessôa de Menezes e. |
| The new ß-lactamases have arisen largely as a consequence of heavy use of new expanded spectrum ß-lactam antibiotics. Health professionals need to be aware of the resistance problems caused by the new enzymes, and know the correct procedures to detect, prevent and control such problems. In this study, a new screening medium called Ceftazidime-Inositol-Vancomycin-Amphotericin B Agar (CIVA) was elaborated and the microbiological performance was evaluated for the detection and presumptive identification of ESBL-producing members of Enterobacteriaceae. It was performed in 126 stool samples from hospitalized patients at Santa Monica Hospital (Vila Velha, ES, Brazil), who had been heavily exposed to broad-spectrum antibiotic combinations. The bacteria were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterobacteriaceae; Culture medium; ESBL. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400006 |
| |
|
|
| Leal,Maria Betania Batista; Baruque-Ramos,Júlia; Hiss,Haroldo; Paz,Marcelo Fossa da; Sakai,Maria Cristina; Vassoler,Umbelina Macedo; Arauz,Luciana Juncioni de; Raw,Isaías. |
| The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest productivity in terms of colony forming units, namely 2.7·10(6) colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BCG; Mycobacterium bovis; Culture medium; Vaccine; Submerged batch cultivation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300013 |
| |
|
|
| Santos,Silvia; Baruque-Ramos,Júlia; Tanizaki,Marta Massako; Lebrun,Ivo; Schenkman,Rocilda Perazzini Furtado. |
| Meningococcal disease is an important cause of death and morbidity throughout the world. Nearly 330,000 cases and 35,000 deaths occur yearly. Neisseria meningitidis, serogroup B strain N.44/89, is prevalent in Brazil. Its outer membrane vesicles (OMV) with iron regulated proteins (IRP) are released to the culture medium and are used as antigen for vaccine production. In order to have knowledge about the kinetic parameters, especially the final OMV concentration values, 20-h batch cultivations were carried out in Catlin medium with iron restriction. Process conditions comprised: 7 L bioreactor, 36ºC, 0.5 atm, overlay air flowrate of 1 L/min, agitation varying from 250 rpm to 850 rpm and dissolved oxygen control set at 10% of saturation condition. Biomass... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neisseria meningitidis; Outer membrane vesicles; Culture medium; Vaccine; Submerged batch cultivation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400016 |
| |
|
|
| Cantarelli,Vlademir Vicente; Inamine,Everton; Brodt,Teresa Cristina Zenobin; Secchi,Carina; Pereira,Fabiana de Souza; Amaro,Maria Cristina; Batalha,Alice Aurora; Ligiero,Simone Dornellas. |
| Stringent quality control protocols must be used in order to guaranty that a particular medium is able to recover all sort of organism that may be present in clinical samples. Our aim was to evaluate an alternative protocol that would allow us to detect medium failure to yield quantitative growth of selected pathogens, and compare with the document M22-A2 from NCCLS. The detection limit of Haemophilus influenzae was significantly different depending on media source. We conclude that for some fastidious microorganisms, quantitative verification of the growth capacity of the culture medium is advised. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quality control; Culture medium; NCCLS. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500003 |
| |
|
| |
|
| |
|
|
| ZOU,Yu; HOU,Xiyan. |
| Abstract Melanin is a natural high molecular weight pigment with the huge application value and development potential in food industry. In the present study, medium composition for melanin production by fungus Auricularia auricula was investigated. Wheat bran extract, l-tyrosine, and CuSO4 were determined to optimize medium composition by response surface methodology with Box–Behnken design. Results indicated that the optimal medium composition was 26.80% (v/v) wheat bran extract, 1.59 g/L l-tyrosine, and 0.11 g/L CuSO4, and the maximum melanin yield was 519.54 mg/L. Melanin production through A. auricula fermentation avoided expensive enzymatic or complicated chemical methods for melanin extraction from tissues of plant or animal, which had the huge... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Optimization; Culture medium; Melanin; Auricularia auricula. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100153 |
| |
|
|
| Castro,Gabriela Fernandes Pepe da Silva de; Rizzo,Roberta Ferreira; Passos,Thaís Souza; Santos,Beatriz Nascimento Corrêa dos; Dias,Daiana da Silva; Domingues,Josiane Roberto; Araújo,Kátia Gomes de Lima. |
| In biotechnological processes, the culture media components are responsible for high costs and exert a strong influence on the cyanobacteria behavior. The objective of this study was to evaluate the Arthrospira platensis growth potential for biomass production under different cultivation conditions using an experimental design. Three factors that are important for cyanobacteria growth were evaluated: sodium bicarbonate (9 to 18 g/l), sodium nitrate (1.25 to 2.5 g/l), and irradiance (20 to 120 µmol photons/m2.s–1). The results showed that the concentration of NaNO3 in the A. platensis medium can be reduced, resulting in increased concentrations of biomass produced. There was a higher biomass production due to the increase in the concentration of NaHCO3 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyanobacteria; Culture medium; Sodium bicarbonate; Sodium nitrate; Irradiance. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100018 |
| |
|
| |
|
|
| Souza,Rafaeli Aparecida Vieira de; Braga,Francyane Tavares; Setotaw,Tesfahun Alemu; Vieira Neto,João; Azevedo,Patrícia Helena de; Azevedo,Virgínia Helena de; Cançado,Geraldo Magela de Almeida. |
| The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Olea europaea; Culture medium; Complex mixture; Tissue culture; Zygotic embryos. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000200016 |
| |
|
|
| Campos,Jonatas da Silva; Bogo,Amauri; Sanhueza,Rosa Maria Valdebenito; Casa,Ricardo Trezzi; Silva,Fabio Nascimento da; Cunha,Isabel Cristina da; Kuhnem Júnior,Paulo Roberto. |
| ABSTRACT: European apple canker (EC) is caused by Neonectria ditissima, a pathogen officially registered as a quarantine pest in 2012. Thirty-five isolates of N. ditissima of different geographical regions of southern Brazil from apple branches showing symptoms of EC were identified by the specific pair primers Ch1 and Ch2 and analyzed concerning the virulence on Gala apple cultivar and morphophysiological characteristics. The disease symptoms were characterized and the isolates compared based on average mycelium growth (AMG), mycelium growth index (MGI), colony color, conidia type, dimensions and growth on potato dextrose agar (PDA), malt agar (AM), and synthetic SNAY (SN) culture media. Nineteen isolates showed the greatest AMG on PDA, forming three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Malus domestica L.; Culture medium; Mycelial and conidial morphophysiology.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500152 |
| |
|
|
| Pérez Ramírez, Jacqueline. |
| En esta investigación, se evaluó el efecto de las diluciones del agua de coco y la adición de biotina sobre el crecimiento de Saccharomyces cerevisiae. En este sentido, la dilución 1:1 adicionada con biotina (µ= 0.21 h-1) y biomasa (3.32 g/L) fue suficiente para dar los requerimientos nutricionales a la cepa estudiada, en comparación con el agua de coco 100% (µ= 0.13 h-1) y biomasa (3.28 g/L) y un medio cultivo sintético (µ= 0.12 h-1) con biomasa de 2.86 g/L. Los nutrientes disponibles en diluciones 1:2 (µ= 0.18 h-1) y biomasa (2.25 g/L), podrían ser considerables para estudios posteriores; no así, las diluciones 1:5 y 1:10 que parecen inadecuadas para el óptimo crecimiento de S. cerevisiae. Se observó el efecto positivo de la biotina sobre el crecimiento... |
|
Palavras-chave: Cepa; Medio de cultivo; Biomasa; Strain; Culture medium; Biomass production; Maestría Tecnológica; Agroindustria. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/643 |
| |
|
|
| Bhatt,Arvind; Stanly,Christine; Keng,Chan Lai. |
| The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alocasia amazonica; A. cuprea; A. robusta; A. longiloba; A. chaii; Culture medium; Acclimatization. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006 |
| |
| Registros recuperados: 27 | |
|
|
|
