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Successful amplification of mitochondrial DNA from dentin of the bottlenose dolphin Tursiops truncatus BABT
Weber,Laura Isabel; Luca,Michelle Jaccottet de; Barreto,André Silva; Souza,Tiago Tolentino de.
Twenty-four teeth of Tursiops truncatus from the collection of the Marine Mammals Laboratory (FURG-RS) were analysed for their potential as DNA source for population genetic and taxonomic studies. All teeth were submitted to decontamination, grinding and demineralisation processes. Dentin powder was weighed for DNA extraction, which was evaluated by the amplification of two mitochondrial genes through PCR using universal primers. Cytochrome-b gene was amplified successfully. PCR products showed concentrations of 3-12 ng/µl for dentin powder, which was positively correlated to the quantity of dentin powder used for DNA extraction. To rule out contamination a SSCP was carried out including positive controls, and samples of humans involved in the analysis....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dolphins; Tursiops; Museum specimens; Dentin; Mitochondrial DNA; Cytochrome-b.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100002
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Phylogenetic relationships and karyotype evolution in the sigmodontine rodent Akodon (2n = 10 and 2n = 16) from Brazil Genet. Mol. Biol.
Silva,Maria José de J.; Patton,James L.; Yonenaga-Yassuda,Yatiyo.
Comparison of G-bands from 2n = 10 and 2n = 16 karyotypes of Akodon revealed tandem fusions, pericentric inversions and Robertsonian rearrangement in autosomes and addition/deletion of constitutive heterochromatin in sex chromosomes. Cytochrome-b sequences indicate that the 2n = 10 karyotype is a new species and show it to be a sister taxon of the 2n = 14, 2n = 15 and 2n = 16 karyotypes. Indeed, this group shows a particular evolutionary situation in which a unique taxonomic unit based on morphological data can be detected, but, karyologically, it can be separated into two groups (2n = 14-15-16 and 2n = 10). Cytochrome-b sequences show a finer resolution, indicating that these four karyotypes represent three molecular entities (2n = 14-15, 2n = 16 and 2n =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Akodon; Cytochrome-b; G-bands; Karyotypical evolution; Phylogeny.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300012
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