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Zhanga,Yong; Min,Qingsong; Xu,Jian; Zhang,Ke; Chen,Shulin; Wang,Haijun; Li,Demao. |
Background: Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. Results: DHA production increased by 47% and reached 5.51 g/L when 4 g malate/L was added during the rapid lipid accumulation stage in shake-flasks culture. Inducing effects of malate was further investigated through the analysis of three kinetic parameters, including specificcell growth rate(μ), specific glucose consumption rate (qGlu)and DHA formation rate (qDHA). DHA concentration was enhanced through a novel fed-batch strategy to a maximum value of 30.7 g/L, giving a yield of 0.103 g DHA/g... |
Tipo: Journal article |
Palavras-chave: DHA; Malate; NADPH; Schizochytrium sp. |
Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100009 |
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Le Nechet, Sandrine; Dubois, Nolwenn; Gouygou, Jean-paul; Berge, Jean-pascal. |
If rays are traditionally fished for their caudal fins, the rest of the body is wasted, except part of the skin that can be transformed into leather. Liver oil of the ray, Himantura bleekeri, was characterized in terms of lipid class composition and fatty acid profile. Liver oil content was high and represented 54% of the liver weight (w/w). Neutral lipids were predominant (92%), major components being triacylglycerols (63%). Other neutral lipids identified were hydrocarbons, sterol esters, mono- and di-acylglycerols, free fatty acids, sterols and glyceryl ethers. Polyunsaturated fatty acids of the n-3 series, namely eicosapentaenoic and docosahexaenoic acids, were high (4% and 16%, respectively). Cholesterol was the major component in the sterol fractions... |
Tipo: Text |
Palavras-chave: Sterol; Squalene; DHA; EPA; PUFA; Lipids; Fatty acids; Ray liver oil. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3129.pdf |
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Palacios, Elena; Racotta, I; Kraffe, E; Marty, Y; Moal, Jeanne; Samain, Jean-francois. |
Seasonal variations in fatty acid composition in several tissues of a 1-year-old population of giant lion's-paw scallop Nodipecten subnodosus were analyzed during gonad development. Samples were taken bimonthly from December 1999 to June 2000, a period in which gonad development was occurring. Fatty acid accumulation in neutral and polar lipids of the female gonad was concomitant to the gonad development and presented a maximum in June. Among essential fatty acids, a specific accumulation was observed only for 20:5n - 3 in the female gonad, as suggested by an increase in its proportion in the neutral and the polar fractions. However, no specific accumulation was observed for 22:6n - 3, as its proportion remained relatively constant in both fractions. We... |
Tipo: Text |
Palavras-chave: Reproduction; Pectinidae; HUFA; EPA; DHA; Arachidonic acid. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-767.pdf |
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Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Lambert, Christophe; Bideau, Antoine; Bidault, Adeline; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe. |
The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte Alexandrium minutum. The purpose of this work was to follow the progressive incorporation of an isotopic label (13CO2) into 11 fatty acids to better understand the fatty acid synthesis pathways in A. minutum. The Dinophyte growth was monitored for 54 h using high-frequency sampling. A. minutum presented a growth in two phases. A lag phase was observed during the first 30 h of development and had been associated with the probable temporary encystment of Dinophyte cells. An exponential growth phase was then observed after t30. A. minutum rapidly incorporated 13C into 22:6n-3, which ended up being the most 13C-enriched... |
Tipo: Text |
Palavras-chave: PUFA synthesis; 22:6n-3; DHA; PKS pathway; Dinophyte; Alexandrium minutum; Encystment. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00654/76562/77688.pdf |
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Campos,Célia Maria Teixeira de; Hamad,Antônio José Simões; Block,Jane Mara; Padilha,José Carlos Fiad; Ogliari,Paulo José; Barreira-Arellano,Daniel; Moreira,Rosana Nogueira Cavalcanti. |
Fatty acids omega--3 and omega--6 composition and specially DHA were determined in freeze-dried chicken embryo eggs with pre-determined incubation periods. Fertile and embryo eggs presented palmitic (23.18 + 0.54%), stearic (7.70 + 0.28%), palmitoleic (3.00 + 0.19%), oleic (36.28 + 0.58%), linoleic (22.18 + 0.34%), linolenic (1.08 + 0.04%), arachidonic (2.04 + 0.03%), docosahexaenoic (0.91 + 0.03%), total omega-3 acids (2.26 + 0.10%) and total omega-6 acids (24.62 + 0.33%). There were no significant differences in total contents of omega-3 fatty acids (p=0.1226) between freeze-dried chicken embryo eggs with different incubation periods (3, 5, 7, 9, and 11 days) and fertile freeze-dried chicken eggs (day 0). However, there were significant differences in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken embryo eggs; Fatty acids omega-3 and omega-6; DHA. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200009 |
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Navarro-García,Gerardo; Gámez-Meza,Nohemí; Medina-Juárez,Luis Angel; Ortega-García,Jesús; Cota-Quiñones,Elizabeth; Ramirez-Suarez,Juan Carlos. |
ABSTRACT: The effect of the addition of natural antioxidants on oxidative stability of ray ( Rhinoptera bonasus) liver oil was evaluated. Different concentrations of rosemary extract, α-tocopherol, and caffeic acid (w/w) were added to the oil. Afterwards, concentration of each antioxidant that gave the highest stability was used in an accelerated stability test (Schaal method). The protective effect was established by the retention in the oil of fatty acids eicosapentaenoic (EPA) and docosahexaenoic (DHA), and concentration of volatile compounds. The best concentrations were: 2.5% of rosemary extract, 0.5% of α-tocopherol, and 0.06% of caffeic acid. Rosemary extract showed to be the product with the greatest ability to retard oxidation, followed by... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ray liver oil; Natural antioxidants; EPA; DHA; Volatile compounds. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100752 |
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