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AFLP analysis of Xanthomonas axonopodis and X. arboricola strains used in xanthan production studies reveal high levels of polymorphism BJM
Mayer,Laurí; Silva,Wladimir Padilha da; Moura,Andrea Bittencourt; Vendruscolo,Claire Tondo.
Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of 14 strains of Xanthomonas arboricola pv. pruni and seven strains of X. axonopodis pv. phaseoli, which are used in xanthan production studies. Relationships identified by the AFLP profiles were assessed for xanthan production capacity, geographical location and host plant. Strains were isolated from 10 different geographic regions in South and Southeast States in Brazil. Data were analyzed for genetic similarity using the Dice coefficient and subjected to UPGMA cluster analysis. A total of 128 AFLP fragments were generated from four primer combinations: EcoRI+C/MseI+0, EcoRI+A/MseI+0, EcoRI+G/MseI+T and EcoRI+G/MseI+A. Of these, 96.1% were polymorphic. X. axonopodis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA fingerprints; Genomic diversity; Pathovar phaseoli; Pathovar pruni.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300026
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