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	Provedor de dados Acta Amazonica (1) Arq. Bras. Med. Vet. Zootec. (1) BABT (1) Scientia Agricola (1) Autor BHERING,Leonardo Lopes (1) Borges,Aline (1) Bressan,Eduardo de Andrade (1) Carvalho,Yanê (1) Chapaval,L. (1) DAL’SASSO,Thaís Carolina da Silva (1) Duarte,F.R. (1) Gomes,J.E. (1) HATTORI,Eric Koiti Okiyama (1) Higuti,llma Hiroko (1) MANGARAVITE,Érica (1) Moon,D.H. (1) Moreira,Mônica (1) Neiva,Ivana Froede (1) Noschang,Juliana (1) OLIVEIRA,Luiz Orlando de (1) Queiroz-Silva,Jurema Rosa de (1) Recchia,Gustavo Henrique (1) Rosa,Mariana Silva (1) TERRA,Vanessa (1) Mais... Palavra-chave DNA isolation (4) Ascorbic acid (1) CTAB (1) Dithiothreitol (1) Genomic DNA (1) Ipomoea batatas (1) Landraces (1) Protocol (1) SSR (1) Staphylococcus aureus (1) Streptococcus (1) Ultrasound (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/report (1) Ano 2020 (1) 2010 (1) 2009 (1) 2008 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment Acta Amazonica MANGARAVITE,Érica; TERRA,Vanessa; HATTORI,Eric Koiti Okiyama; DAL’SASSO,Thaís Carolina da Silva; BHERING,Leonardo Lopes; OLIVEIRA,Luiz Orlando de. ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend... Tipo: Info:eu-repo/semantics/report Palavras-chave: Ascorbic acid; Dithiothreitol; DNA isolation. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400335 An alternative method for Staphylococcus aureus DNA isolation Arq. Bras. Med. Vet. Zootec. Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M.. This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl. Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Genomic DNA; DNA isolation. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004 Methodological variations in the isolation of genomic DNA from Streptococcus bacteria BABT Moreira,Mônica; Noschang,Juliana; Neiva,Ivana Froede; Carvalho,Yanê; Higuti,llma Hiroko; Vicente,Vânia Aparecida. In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility. Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus; DNA isolation; CTAB; Ultrasound. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013 CTAB methods for DNA extraction of sweetpotato for microsatellite analysis Scientia Agricola Borges,Aline; Rosa,Mariana Silva; Recchia,Gustavo Henrique; Queiroz-Silva,Jurema Rosa de; Bressan,Eduardo de Andrade; Veasey,Elizabeth Ann. Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ipomoea batatas; SSR; DNA isolation; Landraces; Protocol. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000400015 Registros recuperados: 4 Primeira ... 1 ... Última

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