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	Provedor de dados 58 (2) Autor Alves,Sydney Hartz (1) Arenhart,Sandra (1) Cueto,Ana Paula (1) Lovato,Luciane Teresinha (1) Padmanabhan,Sriram (1) Pilau,Marciele Ribas (1) Rao,Sneha (1) Sundarrajan,Sudarson (1) Weiblen,Rudi (1) Mais... Palavra-chave DNA virus (2) Antimicrobial activity (1) Condimental herbs (1) Hepatitis B virus (1) RNA virus (1) RecA (1) Thermus thermophilus (1) Verbenaceae (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Antiviral activity of the Lippia graveolens (Mexican oregano) essential oil and its main compound carvacrol against human and animal viruses 58 Pilau,Marciele Ribas; Alves,Sydney Hartz; Weiblen,Rudi; Arenhart,Sandra; Cueto,Ana Paula; Lovato,Luciane Teresinha. Mexican oregano (Lippia graveolens) is a plant found in Mexico and Central America that is traditionally used as a medicinal herb. In the present study, we investigated the antiviral activity of the essential oil of Mexican oregano and its major component, carvacrol, against different human and animal viruses. The MTT test (3-4,5-dimethythiazol-2yl)-2,5-diphenyl tetrazolium bromide) was conducted to determine the selectivity index (SI) of the essential oil, which was equal to 13.1, 7.4, 10.8, 9.7, and 7.2 for acyclovir-resistant herpes simplex virus type 1 (ACVR-HHV-1), acyclovir-sensitive HHV-1, human respiratory syncytial virus (HRSV), bovine herpesvirus type 2 (BoHV-2), and bovine viral diarrhoea virus (BVDV), respectively. The human rotavirus (RV) and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Verbenaceae; Antimicrobial activity; RNA virus; DNA virus; Condimental herbs. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400049 Cloning and high-level expression of Thermus thermophilus RecA in E. coli: purification and novel use in HBV diagnostics 58 Sundarrajan,Sudarson; Rao,Sneha; Padmanabhan,Sriram. ABSTRACT We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. The purified RecA could enhance the polymerase chain reaction (PCR) signals of HBV and improve the detection limit of the HBV diagnosis by real time PCR. The yield of recombinant RecA was ∼35 mg/L, the highest yield reported for a recombinant RecA to date. RecA can be successfully employed to enhance detection... Tipo: Info:eu-repo/semantics/article Palavras-chave: RecA; Hepatitis B virus; DNA virus; Thermus thermophilus. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400848 Registros recuperados: 2 Primeira ... 1 ... Última

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