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| Lima,Consuelo M. R. de; Zanotta,Pedro J. P.; Ricart,Carlos A. O.; Sousa,Marcelo V. de. |
| Enterolobin is a 52.9 kDa, cytolytic, inflammatory and insecticidal protein present in Enterolobium contortisiliquum (Vell.) Morong, a Brazilian large-sized tree. This protein was previously described to occur in different forms of association (monomeric and oligomeric forms). In the current work, we investigated the presence of enterolobin forms of association and hemolytic activity in maturing, germinating and germinated seeds. Immunoblotting analysis revealed the presence of the monomeric form of enterolobin in all phases under study, whereas the dimer was detected in developing seeds in the second half of the maturation period, during germination and in the early post-germination phase. The trimeric form of enterolobin appeared one week after the dimer... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Defense proteins; Hemolytic proteins; Plant defense; Seed germination. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000200008 |
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| Diz,Mariângela S. S.; Carvalho,André O.; Gomes,Valdirene M.. |
| Plants exude a variety of substances through their surface especially of roots and germinating seeds. Some of these released compounds seem to have an inhibitory action against certain pathogens. Lipid transfer proteins (LTPs) are 9 kDa cysteine-rich cationic peptides and are thought to play a role in the protection of plants against microbial infections. The aim of this work was to isolate and determine the molecular mass of a LTP present in the exudates of imbibed cowpea seeds. For exudation induction, 50 seeds were submerged in 50 mL sterile 100 mmol.L-1 Na-acetate buffer, pH 4.5 and shaken at 30 ºC for 24 h. The resulting exudate was subjected to ammonium sulphate fractionation and the pellet formed between 0 and 70 % saturation was dialysed and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Defense proteins; LTP; Seed exudates. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000300007 |
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