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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Chemale,G. (1) Farias,S.S. (1) Gimba,E.R.P. (1) Kayani,Mahmood Akhtar (1) Masood,Nosheen (1) Zaha,A. (1) Mais... Palavra-chave Deletions (2) Actin (1) Cancer (1) Cestode (1) Echinococcus granulosus (1) GSTM1 (1) GSTT1 heterozygous (1) Gene expression (1) Homozygous (1) Promoters (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Protection against laryngeal and pharyngeal carcinoma: Heterozygous vs. homozygous deletions of GSTM1 and GSTT1 Genet. Mol. Biol. Masood,Nosheen; Kayani,Mahmood Akhtar. Deletions in GSTM1 and GSTT1 genes are considered to be a risk factor for cancer development but the exact location of these deletions in the genome was unknown. Three main objectives of the current study were to: (a) identify the boundaries of these deletions in the human genome, (b) screen homozygous (-/-) and heterozygous (+/-) deleted, as well as homozygous present (+/+) individuals using PCR assays, (c) detect associations of pharyngeal (PC) and laryngeal cancer (LC) with the respective genotypes. In total, 102 PC and 92 LC patients were screened and compared with 150 controls. PCR mapping and sequencing revealed a 6 kbp deletion for GSTM1 and a 9 kbp deletion for the GSTT1 gene. The mean age of PC cases was 48.1 (±16.7) years; for LC cases it was... Tipo: Info:eu-repo/semantics/article Palavras-chave: GSTM1; GSTT1 heterozygous; Homozygous; Deletions; Cancer. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100001 Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line BJMBR Gimba,E.R.P.; Chemale,G.; Farias,S.S.; Zaha,A.. We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII... Tipo: Info:eu-repo/semantics/article Palavras-chave: Echinococcus granulosus; Actin; Promoters; Cestode; Gene expression; Deletions. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100004 Registros recuperados: 2 Primeira ... 1 ... Última

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