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| Registros recuperados: 12 | |
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| Simon,Thomas; Tanguy-Royer,Séverine; Royer,Pierre-Joseph; Boisgerault,Nicolas; Frikeche,Jihane; Fonteneau,Jean-François; Grégoire,Marc. |
| Dendritic cells (DCs) are professional antigen-presenting cells involved in the control and initiation of immune responses. In vivo, DCs exposed at the periphery to maturation stimuli migrate to lymph nodes, where they receive secondary signals from CD4+ T helper cells. These DCs become able to initiate CD8+ cytotoxic T lymphocyte (CTL) responses. However, in vitro investigations concerning human monocyte-derived DCs have never focused on their functional properties after such sequential maturation. Here, we studied human DC phenotypes and functions according to this sequential exposure to maturation stimuli. As first signals, we used TNF-α/polyI:C mimicking inflammatory and pathogen stimuli and, as second signals, we compared activated CD4+ T... |
| Tipo: Journal article |
Palavras-chave: Cytotoxic T lymphocytes; Dendritic cells; T memory cell; Sequential maturation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100005 |
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| Pino-Lagos,Karina; Michea,Paula; Sauma,Daniela; Alba,Andrea; Morales,Jorge; Bono,María Rosa; Fierro,Alberto; Rosemblatt,Mario. |
| One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (T REG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect T REG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled T REG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and... |
| Tipo: Journal article |
Palavras-chave: CD4+CD25+ regulatory T cell; Cyclosporine A; Dendritic cells; Transplant tolerance. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300010 |
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| Zheng,Jin; Liu,Qiang; Yang,Jiandong; Ren,Qinyou; Cao,Wei; Yang,Jingyue; Yu,Zhaocai; Yu,Fang; Wu,Yanlan; Shi,Hengjun; Liu,Wenchao. |
| A dendritic cell (DC)-based vaccine strategy could reduce the risk of recurrence and improve the survival of breast cancer patients. However, while therapy-induced apoptosis of hepatocellular and colorectal carcinoma cells can enhance maturation and antigen presentation of DCs, whether this effect occurs in breast cancer is currently unknown. In the present study, we investigated the effect of doxorubicin (ADM)-induced apoptotic MCF-7 breast cancer cells on the activation of DCs. ADM-induced apoptotic MCF-7 cells could effectively induce immature DC (iDC) maturation. The mean fluorescence intensity (MFI) of DC maturity marker CD83 was 23.3 in the ADM-induced apoptotic MCF-7 cell group compared with 8.5 in the MCF-7 cell group. The MFI of DC co-stimulatory... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; Dendritic cells; Immunotherapy; Apoptosis; Doxorubicin. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600006 |
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| Registros recuperados: 12 | |
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