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| Carnegie, Ryan; Meyer, Gary; Blackbourn, Janice; Cochennec, Nathalie; Berthe, Franck; Bower, Susan. |
| The protistan parasite Mikrocytos mackini, the causative agent of Denman Island disease in the oyster Crassostrea gigas in British Columbia, Canada, is of wide concern because it can infect other oyster species and because its life cycle, mode of transmission, and origins are unknown. PCR and fluorescent in situ hybridization (FISH) assays were developed for M mackini, the PCR assay was validated against standard histopathological diagnosis, and a preliminary phylogenetic analysis of the M mackini small-subunit ribosomal RNA gene (SSU rDNA) was undertaken. A PCR designed specifically not to amplify host DNA generated a 544 bp SSU rDNA fragment from M mackini-infected oysters and enriched M. mackini cell isolates, but not from uninfected control oysters.... |
| Tipo: Text |
Palavras-chave: Fluorescent in situ hybridization; PCR validation; Mikrocytos mackini; Denman Island disease. |
| Ano: 2003 |
URL: https://archimer.ifremer.fr/doc/2003/publication-2814.pdf |
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| Hine, P; Bower, S; Meyer, G; Cochennec, Nathalie; Berthe, Franck. |
| An ultrastructural study was carried out on Mikrocytos mackini, the cause of Denman Island disease in Pacific oysters Crassostrea gigas in western Canada. Three forms were identified, quiescent cells (QC), vesicular cells (VC) and endosomal cells (EC), QC occurred in the vesicular connective tissue (VCT), haemocytes (hyalinocytes), adductor and heart myocytes, and extracellularly, They had a central round to ovoid nucleus, <7 cisternae of inactive nuclear membrane-bound Golgi, few vesicles and lysosome-like bodies. VC were rarely extracellular and usually occurred in adductor and heart myocytes, in close association with host cell mitochondria. The contents of the host cell mitochondria appeared to pass through a tubular extension into the cytoplasm of... |
| Tipo: Text |
Palavras-chave: Denman Island disease; Amitochondriate protozoan; Ultrastructure; Crassostrea gigas; Mikrocytos mackini. |
| Ano: 2001 |
URL: https://archimer.ifremer.fr/doc/2001/publication-815.pdf |
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