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Physiological and biochemical changes during desiccation tolerance loss in millet (Pennisetum glaucum L.) seeds Agronomy
Fernandes, Joana Souza; Guimarães, Renato Mendes; Faria, Jose Marcio Rocha; Pereira, Diego de Sousa; Coelho, Stefania Vilas Boas; Oliveira, Ariadne Santos.
The aim of this study was to evaluate the physiological and biochemical changes related to desiccation tolerance loss in millet seeds. The studied points of the germination process were determined according to the seed imbibition curve of the millet hybrid ADRF6010: control (0h), 3h of imbibition, 1 and 3 mm radicle. The seeds were dried on silica gel for 72h at 20°C, followed by pre-humidification at 25°C for 24h. Seed physiological quality was evaluated by electrical conductivity and a germination test, and seed vigor was evaluated with a first germination count and a germination speed index. The experiment was performed in a completely randomized design, and means were compared by the Scott-Knott test at a 5% probability. The enzymatic systems of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation sensitivity; Germination; Proteins; Enzymes.; Agronomy; Dessecação de sementes; Fisiologia de Sementes; Tolerância à dessecação em sementes.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36522
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Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds Anais da ABC (AABC)
SOARES,GIULIANA C.M.; DIAS,DENISE C.F.S.; FARIA,JOSÉ M.R.; BORGES,EDUARDO E.L..
ABSTRACT We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell cycle; Desiccation sensitivity; DNA content; Drying; Heat-stable proteins.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502001
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