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Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects Electron. J. Biotechnol.
Pavlov,Maria Soledad; Eissler,Yoanna; Espinoza,Juan Carlos; Garland,David E; Kuznar,Juan.
A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
Tipo: Journal article Palavras-chave: Digital image analysis; Fluorescence microscope methods; Immunofluorescence; Infectious pancreatic necrosis virus (IPNV); SYBR-Green I.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011
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Root distribution of irrigated grapevine rootstocks in a coarse texture soil of the São Francisco Valley, Brazil Rev. Bras. Frutic.
BASSOI,LUÍS HENRIQUE; GRANGEIRO,LEILSON COSTA; SILVA,JOSÉ ANTONIO MOURA E; SILVA,EMANUEL ELDER GOMES DA.
An experiment was carried out to determine the root distribution of four grapevine rootstocks (Salt Creek, Dogridge, Courdec 1613, IAC 572) in a coarse texture soil of a commercial growing area in Petrolina County, São Francisco Valley, Brazil. Rootstocks were grafted to a seedless table grape cv. Festival, and irrigated by microsprinkler. Roots were quantified by the trench wall method aided by digital image analysis. Results indicated that roots reached 1 m depth, but few differences among rootstocks were found. All of them presented at least 90 % of the roots distributed until 0.6 m depth, with a greater root presence in the first 0.4 m. The upper 0.6 m can be taken into account as the effective rooting depth for soil and water management.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Grapevine; Root system; Digital image analysis.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000100008
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