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	Provedor de dados BJM (1) Braz. J. Vet. Res. Anim. Sci. (1) Autor ASSUMPÇÃO,Mayra Elena Ortiz D’Avila (1) Ferreira,Fernanda Stoduto (1) Horvath,Mariana Bandeira (1) SANTOS,Silvia Edelweiss Crusco dos (1) SATZINGER,Sandra (1) Tondo,Eduardo Cesar (1) VANNUCCHI,Camila Infantosi (1) VISINTIN,José Antonio (1) Mais... Palavra-chave Diluents (2) Dogs (1) Freezing (1) Plating methods (1) Salmonella Enteritidis SE86 (1) Semen (1) Sodium dichloroi-socyanurate (1) Stressed cells (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Comparison of five extenders for canine semen freezing Braz. J. Vet. Res. Anim. Sci. SANTOS,Silvia Edelweiss Crusco dos; VANNUCCHI,Camila Infantosi; SATZINGER,Sandra; ASSUMPÇÃO,Mayra Elena Ortiz D’Avila; VISINTIN,José Antonio. Semen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diluents; Freezing; Semen; Dogs. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000500004 Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure BJM Ferreira,Fernanda Stoduto; Horvath,Mariana Bandeira; Tondo,Eduardo Cesar. The objective of the present study was to assess the growth and the recovery of Salmonella (S.) Enteritidis SE86 in different diluents, culture media and using different plating methods after the exposure to 200 mg/kg sodium dichloroisocyanurate (NaDCC). Before and after NaDCC exposure, SE86 was cultured at 30 °C and 7 °C in the following diluents: Peptone water (P), Saline solution (SaS), Peptone water+Saline solution (P+SaS), Peptone water+Tween 80+Lecithin+Sodium thiosulfate (P+N) and Saline solution+Tween 80+Lecithin+Sodium thiosulfate (SaS+N). The SaS diluent was chosen because it was able to maintain cells viable without growth and was further used for plating SE86 on non selective medium (Tryptic Soy Agar-TSA) and on selective media (Mannitol Lysine... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella Enteritidis SE86; Diluents; Plating methods; Stressed cells; Sodium dichloroi-socyanurate. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300018 Registros recuperados: 2 Primeira ... 1 ... Última

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