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| Sonobe,M.H.; Trezena,A.G.; Guilhen,F.B.; Takano,V.L.; Fratelli,F.; Sakauchi,D.; Morais,J.F.; Prado,S.M.A.; Higashi,H.G.. |
| A method for the screening of tetanus and diphtheria antibodies in serum using anatoxin (inactivated toxin) instead of toxin was developed as an alternative to the in vivo toxin neutralization assay based on the toxin-binding inhibition test (TOBI test). In this study, the serum titers (values between 1.0 and 19.5 IU) measured by a modified TOBI test (Modi-TOBI test) and toxin neutralization assays were correlated (P < 0.0001). Titers of tetanus or diphtheria antibodies were evaluated in serum samples from guinea pigs immunized with tetanus toxoid, diphtheria-tetanus or triple vaccine. For the Modi-TOBI test, after blocking the microtiter plates, standard tetanus or diphtheria antitoxin and different concentrations of guinea pig sera were incubated with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tetanus; Diphtheria; Toxin-binding inhibition test; Toxin neutralization. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100009 |
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| Nascimento,D.V.; Lemes,E.M.B.; Queiroz,J.L.S.; Silva Jr.,J.G.; Nascimento,H.J.; Silva,E.D.; Hirata Jr.,R.; Dias,A.A.S.O.; Santos,C.S.; Pereira,G.M.B.; Mattos-Guaraldi,A.L.; Armoa,G.R.G.. |
| The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently available. In the present study, the genomic DNA of the Corynebacterium diphtheriae Park Williams 8 (PW8) vaccine strain was used as a template for PCR amplification of the dtb gene. After amplification, the dtb gene was cloned and expressed in competent Escherichia coli M15™ cells using the expression vector pQE-30™. The lysate obtained from transformed E. coli cells containing the rDTB PW8 was clarified by centrifugation and purified by affinity chromatography. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragment B; Diphtheria toxin; Diphtheria; Dtb gene; E. coli gene expression; Immobilized metal affinity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500007 |
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| Divino-Goes,K.G.; Moraes-Pinto,M.I. de; Dinelli,M.I.S.; Casagrande,S.T.; Bonetti,T.C.S.; Andrade,P.R.; Weckx,L.Y.. |
| The introduction of routine vaccination against tetanus and diphtheria in Brazil has decreased the incidence and changed the epidemiology of both diseases. We then investigated the prevalence of Corynebacterium diphtheriae carrier status and diphtheria and tetanus immunity in São Paulo, Brazil. From November 2001 to March 2003, 374 individuals were tested for the presence of C. diphtheriae in the naso-oropharynx and of serum diphtheria and tetanus antibodies. Participants were all healthy individuals without acute or chronic pathologies and they were stratified by age as follows: 0-12 months and 1-4, 5-9, 10-14, 15-24, 25-39, 40-59, and ³60 years. Antibodies were assessed using a double-antigen ELISA. C. diphtheriae species were identified by biochemical... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Diphtheria; Tetanus; Corynebacterium diphtheriae; Vaccine; Immunization; Antibody. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200012 |
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| Weckx,L.Y.; Divino-Goes,K.; Lihama,D.M.; Carraro,E.; Bellei,N.; Granato,C.F.H.; de Moraes-Pinto,M.I.. |
| Epidemiological data regarding tetanus and diphtheria immunity in elderly people in Brazil are scarce. During the First National Immunization Campaign for the Elderly in Brazil in April 1999, 98 individuals (median age: 84 years) received one tetanus-dyphtheria (Td) vaccine dose (Butantan Institute, lot number 9808079/G). Inclusion criteria were elderly individuals without a history of severe immunosuppressive disease, acute infectious disease or use of immunomodulators. Blood samples were collected immediately before the vaccine and 30 days later. Serum was separated and stored at -20ºC until analysis. Tetanus and diphtheria antibodies were measured by the double-antigen ELISA test. Tetanus and diphtheria antibody concentrations lower than 0.01 IU/mL were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Elderly; Tetanus; Diphtheria; Immunization. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400012 |
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