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Induction of Fos protein immunoreactivity by spinal cord contusion BJMBR
Del-Bel,E.A.; Borges,C.A.G.; Defino,H.L.A.; Guimarães,F.S..
The objective of the present study was to identify neurons in the central nervous system that respond to spinal contusion injury in the rat by monitoring the expression of the nuclear protein encoded by the c-fos gene, an activity-dependent gene, in spinal cord and brainstem regions. Rats were anesthetized with urethane and the injury was produced by dropping a 5-g weight from 20.0 cm onto the exposed dura at the T10-L1 vertebral level (contusion group). The spinal cord was exposed but not lesioned in anesthetized control animals (laminectomy group); intact animals were also subjected to anesthesia (intact control). Behavioral alterations were analyzed by Tarlov/Bohlman scores, 2 h after the procedures and the animals were then perfused for...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Fos immunohistochemistry; Intermediolateral column neurons; Reticular formation; Area postrema; Dorsal horn; Solitary tract nuclei; Laminectomy; Spinal cord injury.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500006
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