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| Passos,Angela Noronha; Kohara,Valdelene Sayuri; Freitas,Roseli Santos de; Vicentini,Adriana Pardini. |
| Several reports showed outbreaks of histoplasmosis acquired while bat-inhabited caves were visited by tourists, miners or researchers. We evaluated the performance of double immunodifusion (DI) and immunoblotting (IB) assays, employed for the histoplasmosis outbreak elucidation occurred in Vale do Paraíba, São Paulo. The existence of epidemiologic link, four patients with clinical signs suggestive of histoplasmosis and mycological confirmation has made that all 35 individuals involved to the cave visit were subjected to serological evaluation. By DI, we observed reactivity against H. capsulatum antigen in a single serum examined nearly 20 days after exposure to fungal propagules. On the other hand, IB showed reactivity against H and M fractions in 50% of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Histoplasma; Histoplasmosis; Immunoassay; Double immunodiffusion; Immunoblotting. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400028 |
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| Santos-Neto,Joaquim R; Mezencio,Jose M S; Chagas,Aline T A; Michereff-Filho,Miguel; Serrão,José E. |
| Spodoptera frugiperda (J E Smith) is an important pest of several crops, but especially on maize in Brazil. The implementation of biological control measures hinges on the identification of its predators and other natural enemies. As a means of identifying predators, antibodies against S. frugiperda eggs were generated by inoculating rabbits with macerated S. frugiperda eggs, and the production of antibodies against S. frugiperda egg proteins was verifi ed by double immunodiffusion (DID). These antibodies were then utilized in another serological technique, counterimmunoeletrophoresis (CIE), to identify insects that could have ingested S. frugiperda eggs. Macerates of entire insects collected in maize plantations and of individual parts of their digestive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fall armyworm; Natural enemy; Double immunodiffusion; Counterimmunoelectrophoresis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000300016 |
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