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| SILVA,A. L. S. da; MORAES-FERNANDES,M. I.; FERREIRA,A. G.. |
| This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anther culture; Doubled haploids. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200016 |
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| Coelho,Mônica Bossardi; Scagliusi,Sandra Maria Mansur; Lima,Maria Imaculada Pontes Moreira; Consoli,Luciano; Grando,Magali Ferrari. |
| Abstract: The objective of this work was to assess the androgenic response, via microspore culture, of wheat genotypes with different levels of resistance to Gibberella zeae. The number of androgenic embryos per spike, and of green and albino plants was counted for the BRS 179 (moderately resistant), Frontana and Sumai 3 (resistant), and BRS 194, Embrapa 27, and Fielder (susceptible) genotypes. The degree of interference by the Fielder, Pavon 76, and Sumai 3 ovary-donor genotypes, used for co-culture with the microspore cells, was also assessed regarding androgenic response. Induction efficiency ranged from 0.33 embryo per spike for Embrapa 27 to 109.8 embryos for Frontana. Sumai 3 presented the second best response, and Embrapa 27 behaved as highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gibberella zeae; Triticum aestivum; Doubled haploids; Microspore. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500575 |
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