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	Provedor de dados Ciênc. Tecnol. Aliment. (1) Repositório Alice (1) BABT (1) Autor Borsói,Caroline (1) Bresolin,Iara Rocha Antunes Pereira (1) Bresolin,Igor Tadeu Lazzarotto (1) FARINAS, C. S. (1) MIRANDA, E. A. (1) Mazzola,Priscila Gava (1) Poletto,Patrícia (1) RECH FILHO, E. L. (1) ROBIC, G. (1) Silveira,Edgar (1) Silveira,Mauricio Moura da (1) Tambourgi,Elias Basile (1) Zeni,Mara (1) Mais... Palavra-chave Downstream processing (3) Bromelain (1) Enzymatic activity (1) Extraction (1) Juice clarification (1) Pectinase formulation (1) Pectinase stability (1) Pineapple peel (1) Recombinant protein (1) Semente (1) Soja transgênica (1) Solid-state cultivation (1) Soybean seed (1) Therapeutic applications (1) Waste (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Separatas (1) Ano 2015 (1) 2013 (1) 2010 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Transgenic soybean seed as protein expression system: aqueous extraction of recombinant b-glucuronidase. Repositório Alice ROBIC, G.; FARINAS, C. S.; RECH FILHO, E. L.; MIRANDA, E. A.. Tipo: Separatas Palavras-chave: Soja transgênica; Downstream processing; Recombinant protein; Soybean seed; Semente; Extraction. Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/871820 Isolation and purification of bromelain from waste peel of pineapple for therapeutic application BABT Bresolin,Iara Rocha Antunes Pereira; Bresolin,Igor Tadeu Lazzarotto; Silveira,Edgar; Tambourgi,Elias Basile; Mazzola,Priscila Gava. The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Pineapple peel; Downstream processing; Waste; Therapeutic applications; Enzymatic activity. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012 Downstream processing of pectinase produced by Aspergillus niger in solid state cultivation and its application to fruit juices clarification Ciênc. Tecnol. Aliment. Poletto,Patrícia; Borsói,Caroline; Zeni,Mara; Silveira,Mauricio Moura da. In this work, a protocol for the formulation of an enzyme concentrated product to be applied in fruit juice treatment is described. Downstream processing conditions for the recovery and concentration of pectinases produced by the new strain Aspergillus niger LB-02-SF in solid state cultivation were assessed. The solid-liquid ratio in the extraction step of pectinases recovery from the cultivated media was evaluated and the highest activity was obtained with a solid-liquid ratio of 1:10. The crude extract was concentrated by ultrafiltration and the total pectinase (TP) activity was 73.6-fold concentrated in relation to the crude extract, and a final TP titer of 663 U mL–1 was obtained with 73.7% of recovery yield. KCl and different glycerol concentrations... Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state cultivation; Downstream processing; Pectinase stability; Pectinase formulation; Juice clarification. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000200391 Registros recuperados: 3 Primeira ... 1 ... Última

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