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Registros recuperados: 47
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Aspectos del polimorfismo genético en Drosophila pseudoobscura, D. willistoni y D. nebulosa Acta Agron. (Palmira)
Salceda Sacanelles,Víctor Manuel.
En México el polimorfismo cromosómico en poblaciones naturales de Drosophila se estudió en D. pseudoobscura, D. willistoni y D. nebulosa. En la primera, más ampliamente analizada, se reconocen 27 inversiones correspondientes al 67.5% de la variabilidad para esta característica, identificando cuatro razas. Diferentes recolectas detectaron nuevas inversiones que permitieron reestructurar el árbol filogenético para la característica. Con base en el alto número de estas nuevas inversiones y otras evidencias se postuló como arreglo ancestral el Tree Line (TL) y se sugiere como centro de origen de la especie la región en que colindan los estados de Michoacán y Zacatecas. Se encontraron gradientes geográficos para algunas inversiones tanto en localidades...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Polimorfismo cromosómico; Poblaciones naturales; México.
Ano: 2008 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122008000200006
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Inventario taxonómico de drosophilidae (Diptera) en el Parque Nacional Yasuni, Amazonia Ecuatoriana Acta Amazonica
Acurio,Andrea Elizabeth; Rafael,Violeta Lily.
En el Parque Nacional Yasuní, reconocido como un sector de alto endemismo y biodiversidad, ubicado al noroeste de la Amazonía Ecuatoriana se realizó el inventario taxonómico de la familia Drosophilidae. Para la captura de los individuos se utilizaron trampas con atrayente de banano y solución de levadura de cerveza. La identificación taxonómica se realizó usando caracteres morfológicos y la terminalia de los machos. En total se colectaron 7425 individuos clasificados en 34 especies de los géneros: Drosophila Fallén, 1823, Scaptodrosophila Duda, 1923, Neotanygastrella Duda, 1923 y Zaprionus Coquillett, 1901. Seis de estas especies son nuevos registros para el Ecuador: D. araicas Pavan & Nacrur, 1950, D. equinoxialis Dobzhansky, 1946, D. kikkawai...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inventario taxonómico; Drosophila; Neotanygastrella; Nuevos registros; Zaprionus.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000300028
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EFECTO DE DENSIDAD POBLACIONAL DE HUEVOS SOBRE VIABILIDAD Y TIEMPO DE DESARROLLO DE Drosophila melanogaster (DROSOPHILIDAE) Acta biol.Colomb.
FONG,CRISTIAN; DÍAZ,FERNANDO; OSORIO,JULIO; CASTANO,LINA; GONZÁLEZ,FANNY; JURADO,LAURA; CASTILLO,KATHERINE; CÁRDENAS,HEIBER.
Para estimar el efecto de la densidad poblacional de huevos sobre la viabilidad huevoadulto de Drosophila melanogaster se realizaron tres tratamientos en frascos con medio de cultivo agar-banano: a densidad de 10, 50 y 90 huevos. La variable de respuesta fue la proporción de individuos adultos emergidos desde la aparición del primer imago hasta 15 días después de la siembra. Las viabilidades huevo-adulto promedio fueron 0,320, 0,338 y 0,328 para las densidades de 10, 50 y 90 huevos respectivamente. No se evidenciaron diferencias significativas entre los tres tratamientos (p>0.05). Por lo tanto, no se detectó en este estudio influencia de la densidad poblacional de huevos sobre la viabilidad huevo-adulto. Probablemente debido a que la proporción entre el...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Densidad poblacional; Drosophila; Huevos; Viabilidad.
Ano: 2008 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2008000200008
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Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni Anais da ABC (AABC)
Blauth,Monica L.; Bruno,Rafaela V.; Abdelhay,Eliana; Loreto,Elgion L.S.; Valente,Vera L.S..
The P element is one of the most thoroughly studied transposable elements (TE). Its mobilization causes the hybrid dysgenesis that was first described in Drosophila melanogaster. While studies of the P element have mainly been done in D. melanogaster, it is believed that Drosophila willistoni was the original host species of this TE and that P was transposed to the D. melanogaster genome by horizontal transfer. Our study sought to compare the transcriptional behavior of the P element in embryos of D. melanogaster, which is a recent host, with embryos of two strains of D. willistoni, a species that has contained the P element for a longer time. In both species, potential transcripts of transposase, the enzyme responsible for the TE mobilization, were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; D. willistoni hybrid dysgenesis RNAi P element transposable element.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000400007
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Ubiquitous overexpression of a transgene encoding the extracellular portion of the Drosophila Roughest-Irregular Chiasm C protein induces early embryonic lethality Anais da ABC (AABC)
MODA,LIVIA; MACHADO,RICARDO C.; RAMOS,RICARDO GUELERMAN P..
The cell adhesion molecule Rst-irreC is a transmembrane glycoprotein of the immunoglobulin superfamily involved in several important developmental processes in Drosophila, including axonal pathfinding in the optic lobe and programmed cell death and pigment cell differentiation in the pupal retina. As an initial step towards the "in vivo'' functional analysis of this protein we have generated transgenic fly stocks carrying a truncated cDNA construct encoding only the extracellular domain of Rst-IrreC under the transcriptional control of the heat shock inducible promoter hsp70. We show that heat-shocking embryos bearing the transgene during the first 8hs of development lead to a 3-4 fold reduction in their viability compared to wild type controls. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Transgenic organisms; Cell adhesion; Immunoglobulin superfamily.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300011
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"In vivo" toxicity of a truncated version of the Drosophila Rst-IrreC protein is dependent on the presence of a glutamine-rich region in its intracellular domain Anais da ABC (AABC)
MACHADO,RICARDO C.; PEREIRA,RODRIGO N.R.; COSTA,MARA S.A.; RAMOS,RICARDO GUELERMAN P..
The roughest-irregular chiasm C ( rst-irreC) gene of Drosophila melanogaster encodes a transmembrane glycoprotein containing five immunoglobulin-like domains in its extracellular portion and an intracytoplasmic tail rich in serine and threonine as well some conserved motifs suggesting signal transduction activity. In the compound eye, loss-of-function rst-irreC mutants lack the characteristic wave of programmed cell death happening in early pupa and which is essential for the elimination of the surplus interommatidial cells. Here we report an investigation on the role played by the Rst-irreC molecule in triggering programmed cell death. "In vivo" transient expression assays showed that deletion of the last 80 amino acids of the carboxyl terminus produces a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Rst-irreC; Glutamine-rich repeats; Cell death.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000200007
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The balance between GMD and OFUT1 regulates Notch signaling pathway activity by modulating Notch stability Biol. Res.
Glavic,Alvaro; López-Varea,Ana; de Celis,José F.
The Notch signaling pathway plays an important role in development and physiology. In Drosophila, Notch is activated by its Delta or Serrate ligands, depending in part on the sugar modifications present in its extracellular domain. O-fucosyltransferase-1 (OFUT1) performs the first glycosylation step in this process, O-fucosylating various EGF repeats at the Notch extracellular domain. Besides its O-fucosyltransferase activity, OFUT1 also behaves as a chaperone during Notch synthesis and is able to down regulate Notch by enhancing its endocytosis and degradation. We have reevaluated the roles that O-fucosylation and the synthesis of GDP-fucose play in the regulation of Notch protein stability. Using mutants and the UAS/Gal4 system, we modified in developing...
Tipo: Journal article Palavras-chave: Notch; GMD; O-fucosyltranserase; Drosophila.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100004
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The S6 kinase Signaling Pathway In The Control of Development and Growth Biol. Res.
THOMAS,GEORGE.
The discussion will focus on the role of the ribosomal protein S6 kinase (S6K) signaling pathway in the regulation of cell growth and proliferation. Although 40S ribosomal protein S6 phosphorylation was first described 25 years ago (<A HREF="#20">Gressner and Wool, 1974</A>), it only recently has been implicated in the translational up-regulation of mRNAs coding for the components of protein synthetic apparatus (<A HREF="#15">Fumagalli and Thomas, 2000</A>). These mRNAs contain an oligopyrimidine tract at their 5' transcriptional start site, termed a 5'TOP, which has been shown to be essential for their regulation at the translational level (<A HREF="#32">Meyuhas et al., 1996</A>). In parallel, a great deal of...
Tipo: Journal article Palavras-chave: Drosophila; SScells; Diabetes.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200022
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Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli Biol. Res.
Muñoz,Yorka; Fuenzalida,Karen; Bronfman,Miguel; Gatica,Arnaldo; Sepúlveda,Marcelo; Bacigalupo,Juan; Roth,Alejandro D; Delgado,Ricardo.
Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel...
Tipo: Journal article Palavras-chave: Rhabdomere; Drosophila; Microvilli; Phototransduction; TRP channel; Fatty acid analysis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010
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Drosophilids (Insecta, Diptera) of the Paranã Valley: eight new records for the Cerrado biome Biota Neotropica
Mata,Renata Alves da; Roque,Francisco; Tidon,Rosana.
The Paranã Valley, located in the Central Brazil Plateau, within the domain of the Cerrado biome, is frequently cited as a center of endemism and diversity. Nonetheless, this region is poorly known, mainly considering its invertebrate fauna. Here, drosophilid flies were used as biological tools to contribute to a broader inventory, whose goals were mapping the Cerrado biodiversity and identifying areas to be conserved. Three previously unsampled areas in the Paranã Valley were sampled. Amongst the 12,297 specimens collected 45 species were identified, which represents 50% of the drosophilid species previously recorded in the Cerrado. Moreover, this sampling presented eight new occurrences for the biome (Drosophila annulosa, D. calloptera, D. papei, D....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodiversity; Brazilian savanna; Central Brazil Plateau; Conservation; Drosophila; Inventory.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032008000100006
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The maternal JAK/STAT pathway of Drosophila regulates embryonic dorsal-ventral patterning BJMBR
Lopes,E.S.S.; Araujo,H.M..
Activation of NFkappaB plays a pivotal role in many cellular processes such as inflammation, proliferation and apoptosis. In Drosophila, nuclear translocation of the NFkappaB-related transcription factor Dorsal is spatially regulated in order to subdivide the embryo into three primary dorsal-ventral (DV) domains: the ventral presumptive mesoderm, the lateral neuroectoderm and the dorsal ectoderm. Ventral activation of the Toll receptor induces degradation of the IkappaB-related inhibitor Cactus, liberating Dorsal for nuclear translocation. In addition, other pathways have been suggested to regulate Dorsal. Signaling through the maternal BMP member Decapentaplegic (Dpp) inhibits Dorsal translocation along a pathway parallel to and independent of Toll. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: JAK kinase; Drosophila; Embryonic patterning; Hopscotch; NFkappaB.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200006
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Effect of single cell protein as a protein source in Drosophila culture BJM
Uysal,Handan; Aydogan,M. Nuri; Algur,O. Faruk.
The effects of biomass of Phanerochaete chrysosporium at various concentrations (1; 2.5; 5; 10; 25; 50; 75 and 100%) on the growth of Drosophila melanogaster have been investigated. Biomass was used as a protein source instead of corn flour in Standard Drosophila Medium(SDM). It was seen that it causes increments in both body size of larvae and the numbers of offsprings, especially at higher concentrations (50, 75 and 100%). At the application of 100% concentration, metamorphosis accelerated and was completed one day before the control. There were phenotipic abnormalities in both control (0.64%) and applications with low concentrations (0.02-0.19%), while they were not observed at the application of 100% concentration
Tipo: Info:eu-repo/semantics/article Palavras-chave: Single cell protein; Toxicity; Drosophila; Phanerochaete chrysosporium.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400007
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Yeast communities in two Atlantic rain Forest fragments in Southeast Brazil BJM
Pimenta,Raphael S.; Alves,Priscila D. D.; Almeida,Gabriel M. F.; Silva,Juliana F.M; Morais,Paula B.; Corrêa Jr.,Ary; Rosa,Carlos A..
We studied the yeast communities associated with fruits, mushrooms, tree exudates, and flies of the genus Drosophila, in two Atlantic Rain Forest fragments in state of Minas Gerais, Brazil. A total of 456 samples were collected from Rio Doce State Park and 142 from Ecological Station of Universidade Federal de Minas Gerais. From these samples, 608 yeast isolates were obtained, belonging to 71 different species. Among the yeasts isolated from Rio Doce State Park, 17 isolates were recovered from fruits, 12 from mushrooms, 13 from tree exudates, and 299 from Drosophila spp. In the Ecological Station of Universidade Federal de Minas Gerais, 24 isolates were recovered from fruits and 243 from Drosophila spp. Distinct communities of yeast were observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast communities; Drosophila; Fruits; Mushrooms; Tree exudates; Atlantic Rain Forests.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100015
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Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease BJPS
Siddique,Yasir Hasan; Jyoti,Smita; Naz,Falaq.
In the present study we have studied the effect of 25, 50, 75 and 100 µM of luteolin on the transgenic Drosophila expressing human alpha synuclein. The doses of luteolin were established in diet and the PD flies were allowed to feed on it for 24 days. After 24 days of exposure the flies were assayed for climbing assay, oxidative stress markers, caspase-3 & 9 activity and dopamine content. The immunohistochemistry was also performed on the brain sections for the activity of tyrosine hydroxylase. The exposure of luteolin showed a dose dependent delay in the loss of climbing ability and activity, reduction in oxidative stress markers, caspase-3&9 activities and results in an increase in the dopamine content. The results obtained for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Luteolin; Parkinson’s disease; Drosophila; Oxidative stress.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300627
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Tracking the Genetic Effects of Global Warming: Drosophila and Other Model Systems Ecology and Society
Scheiner, Samuel M; National Science Foundation; sscheine@nsf.gov.
Current efforts to study the biological effects of global change have focused on ecological responses, particularly shifts in species ranges. Mostly ignored are microevolutionary changes. Genetic changes may be at least as important as ecological ones in determining species' responses. In addition, such changes may be a sensitive indicator of global changes that will provide different information than that provided by range shifts. We discuss potential candidate systems to use in such monitoring programs. Studies of Drosophila subobscura suggest that its chromosomal inversion polymorphisms are responding to global warming. Drosophila inversion polymorphisms can be useful indicators of the effects of climate change on populations and ecosystems. Other...
Tipo: Peer-Reviewed Reports Palavras-chave: Biodiversity conservation; Biological indicator; Chromosomal inversion polymorphism; Climate change; Drosophila; Evolutionary ecology; Genetic diversity; Global warming; Microevolution..
Ano: 1998
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small ORFs: A new class of essential genes for development Genet. Mol. Biol.
Albuquerque,João Paulo; Tobias-Santos,Vitória; Rodrigues,Aline Cáceres; Mury,Flávia Borges; Fonseca,Rodrigo Nunes da.
Genes that contain small open reading frames (smORFs) constitute a new group of eukaryotic genes and are expected to represent 5% of the Drosophila melanogaster transcribed genes. In this review we provide a historical perspective of their recent discovery, describe their general mechanism and discuss the importance of smORFs for future genomic and transcriptomic studies. Finally, we discuss the biological role of the most studied smORF so far, the Mlpt/Pri/Tal gene in arthropods. The pleiotropic action of Mlpt/Pri/Tal in D. melanogaster suggests a complex evolutionary scenario that can be used to understand the origins, evolution and integration of smORFs into complex gene regulatory networks.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tribolium; Mlpt; Pri; Tarsal-less; Drosophila.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300278
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Enhanced tethered-flight duration and locomotor activity by overexpression of the human gene SOD1 in Drosophila motorneurons Genet. Mol. Biol.
Petrosyan,Agavni; Hsieh,I-Hui; Phillips,John P.; Saberi,Kourosh.
Mutation of the human gene superoxide dismutase (hSOD1) is associated with the fatal neurodegenerative disease familial amyotrophic lateral sclerosis (Lou Gehrig’s disease). Selective overexpression of hSOD1 in Drosophila motorneurons increases lifespan to 140% of normal. The current study was designed to determine resistance to lifespan decline and failure of sensorimotor functions by overexpressing hSOD1 in Drosophila‘s motorneurons. First, we measured the ability to maintain continuous flight and wingbeat frequency (WBF) as a function of age (5 to 50 days). Flies overexpressing hSOD1 under the D42-GAL4 activator were able to sustain flight significantly longer than controls, with the largest effect observed in the middle stages of life. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Behavioral genetics; Superoxide dismutase; Drosophila; Motorneuron; Longevity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100107
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Genetic components affecting embryonic developmental time of Drosophila melanogaster Genet. Mol. Biol.
Nascimento,Jurema Cruz do; Cruz,Ivana Beatrice Mânica da; Monjeló,Luiz Alberto; Oliveira,Alice Kalisz de.
The developmental time of the embryonic stage of Drosophila melanogaster was 21.66% faster and 14.75% slower than controls in populations selected for fast and slow developmental speed, respectively. The genetic model with two main loci with dominant and additive effect added to maternal effect and their relevant interactions can explain 96% of the phenotypic variability in the embryonic developmental time according to 14 crossing progenies involving fast and slow flies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Developmental rate; Embryo; Drosophila.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200008
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Distribution and insertion numbers of transposable elements in species of the Drosophila saltans group Genet. Mol. Biol.
Castro,Juliana P. de; Setta,Nathalia de; Carareto,Claudia Marcia A..
Information about the distribution and insertion numbers of many transposable elements is restricted to few species of Drosophila, although these elements are widely distributed throughout the genus. The aim of this work was to describe the distribution and insertion numbers of four retrotransposons (copia, gypsy, micropia, I) and four transposons (hobo, mariner, Minos and Bari-1) in the saltans group of Drosophila. Our data shows that, except for mariner, all the other elements are widespread within the saltans group and show variable insertion numbers of up to 24 copies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Genomic insertion; Saltans group; Retrotransposons; Transposons.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200029
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Distribution and conservation of the transposable element gypsy in drosophilid species Genet. Mol. Biol.
Herédia,Fabiana; Loreto,Elgion Lúcio S.; Valente,Vera Lúcia S..
In an attempt to understand the dynamics of transposable elements (T'S) in the genome of host species, we investigated the distribution, representativeness and conservation of DNA sequences homologous to the Drosophila melanogaster gypsy retrotransposon in 42 drosophilid species. Our results extended the knowledge about the wide distribution of gypsy in the genus Drosophila, including several Neotropical species not previously studied. The gypsy-like sequences showed high divergence compared to the D. melanogaster gypsy element. Furthermore, the conservation of the restriction sites between gypsy sequences from phylogenetically unrelated species pointed to a more complex evolutionary picture, which includes the possibility of the horizontal transfer events...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Retrotransposon; Gypsy; Southern blot; PCR.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100023
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