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| Registros recuperados: 10 | |
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| Koga,Paula Célia Mariko; Menezes,Caroline Anunciação; Lima,Flávia Afonso; Nara,Júlia Mitico; Magalhães,Caroline Arantes; Cianciarullo,Aurora Marques; Ferreira-Júnior,Jorge Mario da Costa; Trabulsi,Luiz Rachid; Mendes-Ledesma,Meire Roberta Bresciani; Piazza,Roxane Maria Fontes. |
| Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intimin; Phenotypical analysis; Polyclonal antibody; EPEC; EHEC. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002 |
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| Kobayashi,Renata K. T.; Saridakis,Halha Ostrensky; Dias,Angela M.G.; Vidotto,Marilda C.. |
| In this work, the prevalence of enteropathogenic Escherichia coli (EPEC) in children in Londrina-PR, Brazil, was evaluated by means of digoxigenin-labelled DNA probes which identify the plasmid responsible for EPEC adherence factor (EAF), and virulence genes for EPEC as bundle-forming pilus (bfp) and E. coli attaching-effacing factor (eae). In addition, the isolated strains were serotyped and tested for adherence to HEp-2 cells. From 102 children with diarrhoea, 19 strains hybridized with at least one probe, and eleven of them were identified as typical EPEC because they hybridized with the three probes used, showed a localized adherence (LA) pattern, and presented no genes for enterotoxins (ST and LT) or invasion as detected by PCR. Six of the typical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Digoxigenin-labelled DNA probes; Virulence factors; Serotypes; Diarrhoea. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400007 |
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| Dias,Mariana Tavares; Bricio,Silvia Maria Lopes; Almeida,Davi Oliveira; Oliveira,Luiz Antônio Trindade; de Filippis,Ivano; Marin,Victor Augustus. |
| Foodborne disease caused by microorganisms is a problem of public health. Minas soft cheese is a national product manufactured using simple technology; it has high level of acceptance in the country making its production an important economic activity. Many microorganisms may be present in foods including the bacterium Escherichia coli (E. coli). Overall, E. coli is a harmless commensal bacterium; however, some strains may have a pathogenic potential. Several outbreaks of foodborne diseases associated with consumption of contaminated cheese have been reported, and the presence of pathogenic strains of E. coli has increased. The objective of this study was to isolate, evaluate the antimicrobial susceptibility, and characterize, by Multiplex PCR, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Minas soft cheese; EPEC; Antimicrobial. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400017 |
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| Baliere, Charlotte. |
| The contamination of coastal areas by potentially pathogenic enteric bacteria is of concern for the sustainability of some uses, such as shellfish farming, recreational shellfish harvesting and bathing. The contamination of these environments may occur through the land-spreading of livestock wastes, animal feces deposited on pastures, wastewaters from slaughterhouses or from municipal wastewater treatment plants. The presence of these bacteria in coastal environment may present a potential risk to human health. In fact, shellfish-borne outbreaks or human infections may occur by the consumption of shellfish from contaminated areas or the ingestion of water during bathing in contaminated waters, respectively. To date, few studies focusing on the presence and... |
| Tipo: Text |
Palavras-chave: STEC; EPEC; Coquillages; Diversité; Virulence; Persistance; STEC; EPEC; Shellfish; Diversity; Virulence; Persistence. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00312/42322/41653.pdf |
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| Koochakzadeh,A; Askari Badouei,M; Zahraei Salehi,T; Aghasharif,S; Soltani,M; Ehsan,MR. |
| ABSTRACT The aim of this study was to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains and to identify the stx gene types in wild captive and companion birds. In total,657 E. coli isolates from 219 birds belonging to 38 different species were investigated for the presence of STEC and EPEC strains. It was shown that five birds (2.28%) carried strains positive for one or more of the virulence factors investigated. The results indicated that 1.8% (n=4) and 0.45% (n=1) of the birds carried STEC and EPEC strains, respectively. All STEC strains harbored the stx2f and eae genes and this finding reveals the role of other birds, in addition to pigeons, as reservoirs of STEC. The only EPEC strain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EPEC; STEC; Stx2f; Pet birds; Wild birds. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400445 |
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| Cerutti,MF; Vieira,TR; Zenato,KS; Werlang,GO; Pissetti,C; Cardoso,M. |
| ABSTRACT The aim of this study was to investigate the presence of shiga toxin-producing Escherichia coli (STEC) and atypical enteropathogenic Escherichia coli (aEPEC) in frozen chicken carcasses sold at stores in southern Brazil. Typical E. coli colonies were enumerated in 246 chicken carcasses, and the presence of stx1, stx2, eae genes was investigated in their rinse liquid and in E. coli strains isolated from those carcasses. Strains of E. coli were also investigated for the presence of bfp gene. A median of 0.6 cfu.g-1(ranging from <0.1 to 242.7 cfu.g-1) of typical E. coli colonies was found in the carcasses. Shiga toxin-encoding genes (stx1 and stx2) were not detected, indicating that the chicken carcasses were negative for STEC. The intimin protein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken meat; Enteropathogenic Escherichia coli; EPEC; Shigatoxigenic Escherichia coli; STEC. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100310 |
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| Registros recuperados: 10 | |
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