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	Provedor de dados Anais da ABC (AABC) (2) Biocell (1) Autor BORODIN,PAVEL M. (1) Chen,Bo (1) Chen,Lei (1) Chen,Lin (1) Gou,Yuanbing (1) KAFTANOVSKAYA,ELENA (1) KUZNETSOV,SERGEY (1) MATVEEVA,NATALIA (1) MATVEEVA,NATALIA M. (1) MITTMANN,JOSANE (1) SEROV,OLEG (1) SEROV,OLEG L. (1) SEROVA,IRINA A. (1) Song,Ruihua (1) Sun,Jing (1) Wang,Jianmin (1) Yin,Liangjun (1) Zhao,Ling (1) Mais... Palavra-chave ES cells (3) Chromosome biotechnology (1) Conditional knockout (1) Cre (1) Cre/loxP (1) EG cells (1) Fibroblast growth factor receptor-2 (FGFR2) (1) Homologous recombination (1) Hybrid cells (1) MAC (1) Pluripotency (1) Reprogramming (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2006 (1) 2001 (1) 2000 (1) País Brazil (2) Argentina (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Embryonic hybrid cells: a powerful tool for studying pluripotency and reprogramming of the differentiated cell chromosomes Anais da ABC (AABC) SEROV,OLEG; MATVEEVA,NATALIA; KUZNETSOV,SERGEY; KAFTANOVSKAYA,ELENA; MITTMANN,JOSANE. The properties of embryonic hybrid cells obtained by fusion of embryonic stem (ES) or teratocarcinoma (TC) cells with differentiated cells are reviewed. Usually, ES-somatic or TC-somatic hybrids retain pluripotent capacity at high levels quite comparable or nearly identical with those of the pluripotent partner. When cultured in vitro, ES-somatic- and TC-somatic hybrid cell clones, as a rule, lose the chromosomes derived from the somatic partner; however, in some clones the autosomes from the ES cell partner were also eliminated, i.e. the parental chromosomes segregated bilaterally in the ES-somatic cell hybrids. This opens up ways for searching correlation between the pluripotent status of the hybrid cells and chromosome segregation patterns and therefore... Tipo: Info:eu-repo/semantics/article Palavras-chave: ES cells; EG cells; Hybrid cells; Pluripotency; Reprogramming. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000400009 Genetic modification of mammalian genome at chromosome level Anais da ABC (AABC) SEROV,OLEG L.; MATVEEVA,NATALIA M.; SEROVA,IRINA A.; BORODIN,PAVEL M.. The review is concerned with a progress in genetic modification of a mammalian genome in vitro and in vivo at chromosomal level. Recently three new approaches for the chromosome biotechnology have been developed: Using Cre/loxP-system a researcher is able to produce targeted rearrangements of whole chromosomes or their segments or particular genes within the genome, and therefore to modify the set, position and copy number of the endogenous elements of the genome. Mammalian artificial chromosomes (MACs) provide a possibility to introduce into genome relatively large segments of alien chromosome material, either artificially constructed or derived from the genome of different species. Using ES-somatic cell hybrids allows to transfer whole chromosomes or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome biotechnology; Cre/loxP; ES cells; MAC. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300012 Construction of engineered murine Embryonic stem cells with conditional knockout of FGFR2 depending on Cre-loxP Biocell Wang,Jianmin; Song,Ruihua; Chen,Lei; Yin,Liangjun; Chen,Bo; Sun,Jing; Gou,Yuanbing; Zhao,Ling; Chen,Lin. Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot and PCR. Results: The targeting vector with conditional knockout of murine FGFR2... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fibroblast growth factor receptor-2 (FGFR2); Conditional knockout; Homologous recombination; Cre; ES cells. Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200002 Registros recuperados: 3 Primeira ... 1 ... Última

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