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	Provedor de dados BJMBR (2) BJM (2) Arq. Bras. Med. Vet. Zootec. (1) Ciência Rural (1) Autor Almeida,D.F. (2) Alves,A.M.B. (2) Ferreira,L.C.S. (2) Lásaro,M.O. (2) Aleixo,José Antonio (1) Aver,Gladis Paim (1) Cabrera-Crespo,Joaquim (1) Ferreira,Luís Carlos Souza (1) Guillobel,H.C.R. (1) Lasaro,Marcio de Oliveira (1) Lasaro,Melissa Ang-Simões (1) Liaqat,Iram (1) Macedo,C. (1) Marin,J.M. (1) Paneto,B.R. (1) Rodrigues,Juliana Falcão (1) Santo,E. (1) Sbrogio-Almeida,Maria Elisabete (1) Schocken-Iturrino,R.P. (1) Mais... Palavra-chave ETEC (6) CFA/I fimbriae (2) Escherichia coli (2) Antimicrobial resistance (1) Asialo-GM1 (1) Biofilm formation (1) Brazil (1) CELISA (1) CFA/I (1) CFA/II (1) CS2 (1) Cheese (1) DNA vaccine (1) DNA vaccines (1) Diarrheal disease (1) Dsc19CotD(His)6 (1) ELISA assays (1) EPEC (1) Foods (1) Heat-labile toxin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (6) Ano 2012 (1) 2007 (2) 1999 (2) 1996 (1) País Brazil (6) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil Arq. Bras. Med. Vet. Zootec. Paneto,B.R.; Schocken-Iturrino,R.P.; Macedo,C.; Santo,E.; Marin,J.M.. The occurrence of toxigenic Escherichia coli in raw milk cheese was surveyed in Middle Western Brazil. Fifty samples of cheese from different supermarkets were analyzed for E.coli. The isolates were serotyped and screened for the presence of verotoxigenic E. coli (VTEC) and enterotoxigenic E. coli (ETEC) by Polymerase Chain Reaction (PCR). The susceptibility to thirteen antimicrobial agents was evaluated by the disk diffusion method. E.coli were recovered from 48 (96.0%) of the samples. The serogroups identified were O125 (6.0%), O111 (4.0%), O55 (2.0%) and O119 (2.0%). Three (6.0%) and 1(2.0%) of the E.coli isolates were VTEC and ETEC, respectively. Most frequent resistance was observed to the following antimicrobials: cephalothin (60.0%), nalidixic acid... Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Cheese; VTEC; ETEC; Antimicrobial resistance. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200035 New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin BJMBR Alves,A.M.B.; Lásaro,M.O.; Almeida,D.F.; Ferreira,L.C.S.. Stimulation of the mammalian immune system by administration of plasmid DNA has been shown to be an important approach for vaccine development against several pathogens. In the present study we investigated the use of DNA vaccines to induce immune responses against an enteric bacterial pathogen, enterotoxigenic Escherichia coli (ETEC). Three plasmid vectors encoding colonization factor antigen I (CFA/I), an ETEC fimbrial adhesin, were constructed. Eukaryotic cells transfected with each of these plasmids expressed the heterologous antigen in different compartments: bound to the cytoplasmic membrane (pRECFA), accumulated in the cytoplasm (pPolyCFA) or secreted to the outside medium (pBLCFA). BALB/c mice were intramuscularly (im) inoculated with purified... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccines; ETEC; Diarrheal disease; CFA/I fimbriae. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200011 New vaccine strategies against enterotoxigenic Escherichia coli: II: Enhanced systemic and secreted antibody responses against the CFA/I fimbriae by priming with DNA and boosting with a live recombinant Salmonella vaccine BJMBR Lásaro,M.O.; Alves,A.M.B.; Guillobel,H.C.R.; Almeida,D.F.; Ferreira,L.C.S.. The induction of systemic (IgG) and mucosal (IgA) antibody responses against the colonization factor I antigen (CFA/I) of enterotoxigenic Escherichia coli (ETEC) was evaluated in mice primed with an intramuscularly delivered CFA/I-encoding DNA vaccine followed by two oral immunizations with a live recombinant Salmonella typhimurium vaccine strain expressing the ETEC antigen. The booster effect induced by the oral immunization was detected two weeks and one year after the administration of the DNA vaccine. The DNA-primed/Salmonella-boosted vaccination regime showed a synergistic effect on the induced CFA/I-specific systemic and secreted antibody levels which could not be attained by either immunization strategy alone. These results suggest that the combined... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccine; ETEC; CFA/I fimbriae; Salmonella bivalent vaccine. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200014 Biofilm formation and binding specificities of CFA/I, CFA/II and CS2 adhesions of Enterotoxigenic Escherichia coli and CfaE-R181A mutant BJM Liaqat,Iram. Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim formation, colonization and host cell adhesions. As a first step, we have determined the biofilm capability of ETEC expressing various types of pili (CFA/I, CfaE-R181A mutant/ CfaE tip mutant, CFA/II and CS2). Further, enzyme-linked immunosorbent assay (ELISA) assay were developed to compare the binding specificity of CFA/I, CFA/II (CS1 - CS3) and CS2 of ETEC, using extracted pili and piliated bacteria. CFA/II strain (E24377a) as well as extracted pili exhibited significantly... Tipo: Info:eu-repo/semantics/article Palavras-chave: ETEC; CFA/I; CFA/II; CS2; Biofilm formation; ELISA assays; Asialo-GM1; R181-cotD; Dsc19CotD(His)6. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300018 Evaluation of experimental conditions for quantification of LT produced by human derived enterotoxigenic Escherichia coli strains BJM Lasaro,Melissa Ang-Simões; Rodrigues,Juliana Falcão; Cabrera-Crespo,Joaquim; Sbrogio-Almeida,Maria Elisabete; Lasaro,Marcio de Oliveira; Ferreira,Luís Carlos Souza. The heat-labile toxin (LT) is a key virulence-associated factor associated with the non-invasive secretory diarrhea caused by enterotoxigenic Escherichia coli (ETEC) strains either in humans or domestic animals. Several LT detection methods have been reported but quantification of the toxin produced by wild-type ETEC strains is usually performed by the GM1 ganglyoside enzyme-linked immunosorbent assay (GM1 ELISA). In this study we conducted the optimization of an alternative LT-quantification method, the antibody-capture ELISA (cELISA). Detailed analysis of the appropriate dilutions of capture and detecting LT-specific antibodies significantly improved the sensitivity of the method. Additionally, testing of different LT extraction techniques indicated that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Heat-labile toxin; LT; ETEC; CELISA. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300012 Prevalence of enteropathogenic and enterotoxigenic Escherichia Coli in foods of animal origin in southern Brazil Ciência Rural Aleixo,José Antonio; Aver,Gladis Paim. Prevalence of Escherichia coli in foods of animal origin from the city of Pelotas, Brazil, was determined. The ocurrence of enterotoxigenic (ETEC) and classic enteropathogenic (EPEC) serogroups among E. coli isolates was determined. E. coli was isolated from 68% of the food samples surveyed. Of 36 food samples tested, 11(30%) and 24(66%) were positive for EPEC and ETEC strains respectively. However, of 187 E. coli isolates tested, 30(16%) were EPEC compared to 75(40%) which were ETEC. The antibiotic resistance pattern revealed that the isolates were highly sensitive to all antibiotics tested. Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; EPEC; ETEC; Foods; Brazil. Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000200013 Registros recuperados: 6 Primeira ... 1 ... Última

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