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Is Malassezia nana the main species in horses’ ear canal microbiome? BJM
Aldrovandi,Ana Lúcia; Osugui,Lika; Coutinho,Selene Dall’ Acqua.
ABSTRACT The objective of this study was to characterize genotypically Malassezia spp. isolated from the external ear canal of healthy horses. Fifty-five horses, 39 (70.9%) males and 16 (29.1%) females, from different breeds and adults were studied. External ear canals were cleaned and a sterile cotton swab was introduced to collect cerumen. A total of 110 samples were cultured into Dixon medium and were incubated at 32 °C for up to 15 days. Macro- and micromorphology and phenotypic identification were performed. DNA was extracted, strains were submitted to polymerase chain reaction technique, and the products obtained were submitted to Restriction Fragment Length Polymorphism using the restriction enzymes BstCI and HhaI. Strains were sent off to genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malassezia nana; Malassezia slooffiae; Microbiome; Horses; Ear canal.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300770
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