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| Ruiz López,Salvador; Souza,José Camisão de; Zaraza González,Javier; De Ondiz Sánchez,Aitor; Romero-Aguirregomezcorta,Jon; Carvalho,Rafaela Rodrigues de; Rath,Detlef. |
| The objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were matured in vitro for 24 hours and fertilized with frozen-thawed sex sorted or unsorted sperm from the same ejaculate. Fertilization was achieved by two experimental protocols: 1) short gamete co-incubation length: 4, 8 and 12 hours; and 2) long gamete co-incubation length: 18 and 24 hours. After in vitro fertilization, ova were fixed and stained to identify early fertilization events. Sperm penetration,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine IVF; Co-incubation length; Early fertilization; Ovum pick up; Sex-sorted sperm. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013001000005 |
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