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| Neves,M.M.; Heneine,L.G.D.; Henry,M.. |
| The aim of this study was to evaluate the use of low concentrations of natural and lyophilized low density lipoprotein (LDL) from hen's egg yolk for cryopreservation of canine semen. Different ammonium sulphate concentrations were tested to extract LDL from egg yolk. The yolk was centrifuged, and LDL was isolated using 10, 20, 40, 45, or 50% ammonium sulphate solution (ASS). The LDL-rich floating fraction was collected for chemical characterization. Dry matter content was lowest (P<0.05) in the LDL extracted with the 50% ASS. The purification of LDL increased in association with increasing ammonium sulphate concentrations. SDS-PAGE showed that the 50% ASS solution yielded a purer fraction of LDL from egg yolk. For semen cryopreservation, TRIS extender... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egg Yolk; LDL extraction; Ammonium sulphate; Dog; Semen. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300769 |
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| MORI,Agnes Veridiana; MENDONÇA Jr.,Cássio Xavier de; WATANABE,Claudio. |
| The purpose of this study was to evaluate the effect of cholestyramine and lovastatin, lipid-lowering agents, upon egg quality, reproductive performance, plasma lipids and egg yolk cholesterol levels of Shaver laying hens. Twenty-six-weeks-old hens were fed basal diet without animal products containing 0.2% cholestyramine (COL1), 0.3% cholestyramine (COL2) or 0.005% lovastatin (LOV) for 6 weeks. It was observed that the supplementation of the drugs did not impair albumen and shell quality. Hen performance was not adversely affected, with the exception of the significant reduction (p < 0.05) in egg weights. No significant changes were observed on plasma lipids, and egg yolk cholesterol remained unchanged with the addition of the drugs. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cholesterol; Cholestyramine; Lovastatin; Egg Yolk; Chickens. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000100015 |
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