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In vivo biocompatibility of nanostructured Chitosan/Peo membranes Arq. Bras. Med. Vet. Zootec.
Vulcani,V.A.S.; Franzo,V.S.; Rabelo,R.E.; Rabbers,A.S.; Assis,B.M.; D'Ávila,M.A.; Antoni,S.M.B..
Electrospinning is a technique that allows the preparation of nanofibers from various materials. Chitosan is a natural and abundant easily obtained polymer, which, in addition to those features, proved to be biocompatible. This work used nanostructured chitosan and polyoxyethylene membranes as subcutaneous implants in Wistar rats to evaluate the biocompatibility of the material. Samples of the material and tissues adjacent to the implant were collected 7, 15, 30, 45 and 60 days post-implantation. Macroscopic integration of the material to the tissues was observed in the samples and slides for histopathological examination that were prepared. It was noticed that the material does not stimulate the formation of adherences to the surrounding tissues and that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanotechnology; Biomaterials; Electrospinning.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401039
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Nanofibrous Scaffolds for Tissue Engineering Applications BABT
Jaiswal,Amit.
The discipline of tissue engineering opens up the ways for repair and regenerate damaged organs and tissues. In the current work biomimetic nanofibrous scaffolds were fabricated by electrospinning. Poly-L-lactic acid (PLLA) was blended with collagen and gelatin to fabricate PLLLA-collagen and PLLA-gelatin fibrous scaffolds respectively. Pure PLLA and gelatin scaffolds served as controls. All the scaffolds displayed randomly oriented smooth fibers studied by SEM. Surface topography and roughness were studied by AFM and surface contact angle was also measured for all the fabricated scaffolds. Surface roughness was found to be higher in collagen and gelatin blended scaffolds in comparison to PLLA scaffold. Blending of collagen and gelatin reduced the surface...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrospinning; Biomimetic; Tissue Engineering.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100602
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Viability of mesenchymal stem cells during electrospinning BJMBR
Zanatta,G.; Steffens,D.; Braghirolli,D.I.; Fernandes,R.A.; Netto,C.A.; Pranke,P..
Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanotechnology; Tissue engineering; Electrospinning; Stem cells.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200006
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Amphotericin-B and vancomycin-loaded chitosan nanofiber for antifungal and antibacterial application BJPS
Karimi,Soroush; Moradipour,Pouran; Azandaryani,Abbas Hemati; Arkan,Elham.
In the present study, a mucoadhesive non-woven fiber mat (d= 116 nm) was fabricated by the electrospinning method using chitosan (80% Wt), polyethylene oxide (10% Wt), cysteine (4% Wt) and drugs (6% Wt), respectively. In addition, a comparative study was conducted to define effect of drugs and mucoadhesive agent on the nanofiber formation. FTIR, SEM, DSC and DMA were used to investigate the chemical and physical properties of the nanofibers. In vitro release of the drugs was assessed over a 48-hour period by the total immersion method. Release data were fitted to kinetic models, including the zero-order, first-order, Higuchi matrix, and Hixson-Crowell. Zone inhibition investigations were used to describe the inhibition content of vancomycin and amphotericin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mucoadhesive; Vancomycin; Amphotericin-B; Electrospinning; Aphthous; Chitosan.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100521
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Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning: a study of antimicrobial activity BJPS
Segala,Karen; Nista,Silvia Vaz Guerra; Cordi,Lívia; Bizarria,Maria Trindade Marques; Ávila Júnior,José de; Kleinubing,Sirlene Adriana; Cruz,Deborah Cristina; Brocchi,Marcelo; Lona,Liliane Maria Ferrareso; Caballero,Nelson Eduardo Durán; Mei,Lucia Helena Innocentini.
abstract This study examines the antimicrobial activity of silver nanoparticles incorporated into nanostructured membranes made of cellulose acetate (CA) and blends of chitosan/poly-(ethylene oxide, CTS/PEO) and prepared by electrospinning. The formation of chemically synthesized Ag nanoparticles (AgNPs) was monitored by UV-visible spectroscopy (UV-Vis) and characterized by transmission electron microscopy (TEM). The size distribution of the AgNPs was measured by dynamic light scattering (DLS), with an average size of approximately 20 nm. The presence of AgNPs on the surface of electrospun nanofibers was observed by field emission electron microscopy (FEG) and confirmed by TEM. The antimicrobial activity of AgNPs incorporated into nanostructured membranes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrospinning; Silver nanoparticles/antibacterial activity; Cellulose acetate; Chitosan.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400911
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Preparation and characterization of naftifine-loaded poly(vinyl alcohol)/sodium alginate electrospun nanofibers BJPS
Esentürk,İmren; Balkan,Timuçin; Güngör,Sevgi; Saraç,Sezai; Erdal,Meryem Sedef.
In this study, naftifine (a topical antifungal drug) loaded poly(vinyl) alcohol (PVA)/sodium alginate (SA) nanofibrous mats were prepared using the single-needle electrospinning technique. The produced nanofibers were crosslinked with glutaraldehyde (GTA) vapor. The morphology and diameter of the electrospun nanofibers were studied by scanning electron microscopy (SEM). SEM images showed the smoothness of the nanofibers and indicated that the fiber diameter increased with crosslinking and drug loading. Atomic force microscopy (AFM) images confirmed the uniform production of the scaffolds, and elemental mapping via energy dispersive X-ray spectroscopy (EDS) showed the uniform distribution of the drug within the nanofibers. An attenuated total reflectance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Naftifine; Nanofibers; Electrospinning; Topical drug delivery.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100606
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