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Transformación genética de cebolla (Allium cepa L.) mediante biobalística Colegio de Postgraduados
Quintana Sierra, María Elena.
El objetivo de la presente investigación fue desarrollar un protocolo de transformación genética de cebolla mediante biobalística, para lo cual fue necesario establecer un sistema eficiente de regeneración de cebolla in vitro. La metodología desarrollada permite la regeneración de plantas de las variedades Cristal y El Toro. La variedad Cristal mostró mayor porcentaje de explantes con plantas y mayor número de plantas por explante. Se obtuvieron plantas después de 16 semanas de haber iniciado el cultivo y éstas formaron microbulbos, los cuales alcanzaron hasta 3 cm de diámetro cuando se transplantaron en invernadero. La respuesta in vitro obtenida superó a la reportada por otros autores. Mediante el sistema de bombardeo de alta presión, las...
Tipo: Tesis Palavras-chave: Allium cepa; Callos embriogénicos; Cultivo de tejidos; Cotransformación; Doctorado; Genética; Onion; Embryogenic callus; Tissue culture; Cotransformation.
Ano: 2009 URL: http://hdl.handle.net/10521/1539
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Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants Electron. J. Biotechnol.
Ramírez-Villalobos,Maribel; de García,Eva.
The purposes of this work were to obtain embryogenic cell suspensions (ECS) from scalps and to regenerate plants of the banana CIEN-BTA-03. Shoot apexes were grown in the scalp-induction medium of Murashige and Skoog plus BA and IAA, following four diverse treatments. The first two, ME22 and ME25, were solid media supplemented with (mg L-1) 22.7 BA plus 0.192 IAA, and 25 BA plus 0.217 IAA, respectively, all containing 1.8 g L-1 of phytagel, and subcultures were performed monthly and bimonthly over 16 months. The other two treatments, IT22 and IT25, resembled ME22 and ME25 but consisted in temporary immersion for four months without subcultures, followed by two months in solid media. The scalps were grown in callus-induction medium and embryogenic calluses...
Tipo: Journal article Palavras-chave: Bananas; Embryogenic callus; Embryogenic cell suspension; Musa sp; Plant regeneration; Scalp; Secondary somatic embryogenesis.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500006
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Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion Horticultura Brasileira
Sivanesan,Iyyakkannu; Kyoung,Kim E; Kyoung,Kim M; Young,Ko E; Park,Se W.
ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa; Auxin; Embryogenic callus; In vitro propagation; Picloram..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000400441
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ปัจจัยต่างๆ ที่มีผลต่อการถ่ายยีนเข้าสู่ปาล์มน้ำมัน Thai Agricultural
Potjamarn Suraninpong; Supawadee Tawaro; Sompong Te-chato.
Oil palm is a highly producing oil plant. The most cultivation area is in the southern part of Thailand. Expended area of oil palm in the others part needs suitable variety. Thus, the object of this study was to improvement of oil palm via gene transfer technique. Embryogenic callus inducing from young leaves of high yield oil palm were cultured on various kinds and concentrations of antibiotics for screening of suitable concentration using for gene transformation. The result showed that hygromycin, kanamycin and phosphinotricin at 20, 200 and 5 mg/l, respectively, were suitable for transformed selection. Cefotaxime concentration at 200 mg/l was suitable for killing of agrobacterium. The study of inoculation time, co-culture time and co-culture...
Tipo: Collection Palavras-chave: Agrobacterium tumefaciens; Oil palm; Gene transfer; Embryogenic callus; Antibiotic; ปาล์มน้ำมัน; การถ่ายยีน; สารปฏิชีวนะ; เอ็มบริโอเจนิคแคลลัส; ระดับความเข้มข้น; แถบดีเอ็นเอ; ใบอ่อน.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5166
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