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Establishment of a pheasant (Phasianus colchicus) spermatogonial stem cell line for the production of interspecies germ line chimeras Electron. J. Biotechnol.
Kim,Jeong Hyun; Sharma,Neelesh; Kim,Sung Woo; Sodhi,Simrinder Singh; Ghosh,Mrinmoy; Kim,Nam Eun; Mongre,Raj Kumar; Oh,Sung Jong; Jeong,Dong Kee.
Background Spermatogonial stem cells (SSCs) are important for the production of interspecies germ line chimeras. The interspecies germ cell transfer technique has been suggested as a way to conserve endangered birds. Our objective was to develop a technique for restoring endangered birds by developing interspecies germ line chimeras between pheasant (Phasianus colchicus) and chicken (Gallus gallus) with SSCs. Results SSCs were isolated from the surgically removed testis of a pheasant. Growth conditions for pheasant SSCs were established by co-culturing STO (SIM mouse embryo-derived thioguanine and ouabain resistant) cells and pheasant SSCs. The colony-forming cells divided and proliferated stably to yield an established SSC line. Pheasant SSCs showed...
Tipo: Journal article Palavras-chave: Endangered birds; In vitro culture; Recipient chicken embryos; SSC transplantation.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500004
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DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser Genet. Mol. Biol.
Maia,Thais Augusta; Vilaça,Sibelle Torres; Silva,Luciana Resende da; Santos,Fabricio Rodrigues; Dantas,Gisele Pires de Mendonça.
Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Noninvasive samples; Genetic diversity; Endangered birds; Mergus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808
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The importance of protected areas for conservation of bare-faced curassow (Crax fasciolata spix, 1825) (Galliformes: Cracidae) in the São Paulo State, Brazil Biota Neotropica
Gomes,Ana Paula Nascimento; Fornitano,Larissa; Costa,Rômulo Theodoro; Angeli,Thaís; Morais,Kimberly Danielle Rodrigues de; Olifiers,Natalie; Bianchi,Rita de Cassia.
Abstract The Bare-faced Curassow Crax fasciolata (Cracidae:Cracinae) is a vulnerable species that was classified as critically endangered by the São Paulo State -Brazil, in 2008. Specialists recommended "searches for areas where there may be a few remaining populations and protection of the last remaining semideciduous forest in the State". Protected areas were investigated in the Cerrado and semideciduous forest in São Paulo State by camera trapping to evaluate the presence of the Bare-faced Curassow. Eight protected areas in Cerrado biome were investigated, but the Bare-faced Curassows was recorded only at Furnas do Bom Jesus State Park (2,069 ha), São Paulo State despite the large sampling effort in larger protected areas such as the Santa Bárbara...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Camera trap; Conservation; Endangered birds.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032018000300211
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