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Inconsistency of the biological control of Meloidogyne incognita race 2 in melon by endophytic bacteria Horticultura Brasileira
Medeiros,Jeane E de; Mariano,Rosa de LR; Pedrosa,Elvira MR; Silveira,Elineide B da.
We obtained 61 rhizobacterium isolates from rhizosphere soil samples collected in melon commercial fields located in Mossoró, Rio Grande do Norte State, Brazil. These isolates, along with 56 endophytic bacteria from the Collection of Cultures of the Plant Bacteriology Laboratory of the Universidade Federal Rural de Pernambuco, were tested for controlling Meloidogyne incognita race 2 in melon. To infest the soil with nematodes, 1000 eggs of Meloidogyne incognita race 2 per plant were placed in pots where seedlings of the yellow-type melon, cultivar AF 682, were growing for 10 days. Two days before, 20 mL of bacterial suspension (0.7 OD570nm) were poured into each pot. After 60 days, fresh root biomass, gall index, egg mass, and the nematode reproduction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meloidogyne; Cucumis melo; Rhizobacteria; Endophytic bacteria; Management.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362009000300010
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Potencial agrícola de bactérias endofíticas obtidas de bambu Guadua spp. Infoteca-e
VALE, S. M. L. S. do; VALE, P. A. A. do; CARVALHO, C. M.; SIVIERO, A..
Esta pesquisa teve por objetivo caracterizar as bactérias endofíticas de Guadua spp. e estimar o seu potencial de uso agrícola como solubilizados de fosfato e fixação de nitrogênio.
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Sustancias de crecimiento vegetal; Solubilización.; Fijación del nitrógeno; Bactéria endofítica; Endophytic bacteria; Cultivo in vitro; Guadua chaparensis; Guadua weberbeauri; Bambu; Fosfato.; Solubilização; Estimulante de Crescimento Vegetal; Cultura In Vitro; Fixação de Nitrogênio; Biofertilizante; Nitrogen fixation; Phosphates.; Bamboos; Guadua; Plant growth substances; In vitro culture; Solubilization.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1119043
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Meios de cultura específicos para o isolamento de bactérias endofíticas que fixam N2 atmosférico. Infoteca-e
BALDANI, V. L. D.; BALDANI, J. I.; DÖBEREINER, Johanna..
bitstream/CNPAB-2010/27137/1/cot012.pdf
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Bactéria endófitica; Endophytic bacteria; Atmospheric nitrogen.; Meio de Cultura; Nitrogênio Atmosférico.; Culture media..
Ano: 1996 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/623430
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Hologenome theory supported by cooccurrence networks of species-specific bacterial communities in siphonous algae (Caulerpa) ArchiMer
Aires, Tania; Moalic, Yann; Serrao, Ester; Arnaud-haond, Sophie.
The siphonous algae of the Caulerpa genus harbor internal microbial communities hypothesized to play important roles in development, defense and metabolic activities of the host. Here, we characterize the endophytic bacterial community of four Caulerpa taxa in the Mediterranean Sea, through 16S rRNA amplicon sequencing. Results reveal a striking alpha diversity of the bacterial communities, similar to levels found in sponges and coral holobionts. These comprise (1) a very small core community shared across all hosts (< 1% of the total community), (2) a variable portion (ca. 25%) shared by some Caulerpa taxa but not by all, which might represent environmentally acquired bacteria and (3) a large (> 70%) species-specific fraction of the community,...
Tipo: Text Palavras-chave: Endophytic bacteria; Bacterial community; Cooccurrence network; Modularity; Coevolution.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00277/38800/37699.pdf
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Growth promotion of common bean and genetic diversity of bacteria from Amazon pastureland Scientia Agricola
Ferreira,Linnajara de Vasconcelos Martins; Carvalho,Fernanda de; Andrade,Júlia Fonseca Colombo; Moreira,Fatima Maria de Souza.
ABSTRACT: A significant number of bacterial species, particularly in the rhizosphere, may benefit plant growth and development. This group of bacteria is known as plant growth-promoting rhizobacteria (PGPR). This study identified genetically isolates of common bean nodules used to trap bacteria from Amazon pastureland and investigated their capacity of nodulating and promoting growth of common bean when inoculated or co-inoculated with CIAT899 strain (Rhizobium tropici). Two experiments were carried out in a greenhouse, in axenic conditions, using the common bean cultivar Talismã. In the first experiment, 56 PGPR strains were evaluated individually regarding growth promotion and nodulation. In the second experiment, inoculation of seven PGPR strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Co-inoculation; Endophytic bacteria; Rhizobia.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600461
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Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a Scientia Agricola
Melo,Flávia Mandolesi Pereira de; Fiore,Marli Fátima; Moraes,Luiz Alberto Beraldo de; Silva-Stenico,Maria Estela; Scramin,Shirlei; Teixeira,Manoel de Araújo; Melo,Itamar Soares de.
In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESI-MS/MS; Pumilacidin; Endophytic bacteria; Bioautography.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002
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Bioprospecting and selection of growth-promoting bacteria for Cymbidium sp. orchids Scientia Agricola
Gontijo,Júlia Brandão; Andrade,Gracielle Vidal Silva; Baldotto,Marihus Altoé; Baldotto,Lílian Estrela Borges.
ABSTRACT: Inoculants containing bacteria which promote growth in plants can increase productivity and both the economic and the environmental cost in plant crop systems. Similarly, in the flower and ornamental plant sector, the use of diazotrophic bacteria is a promising approach for improving orchid propagation from tissue culture to the ex vitro environment. We isolated diazotrophic bacteria from the roots and leaves of Cymbidium sp. The isolates were used to inoculate Cymbidium sp. plantlets during acclimatization in the nursery. After 150 days, plants were collected and their morphological and nutritional characteristics assessed. Eight bacterial strains were isolated containing traits that promote plant growth: Bacillus thuringiensis, Burkholderia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Orchidaceae; Diazotrophic bacteria; Phosphate-solubilizing bacteria; Endophytic bacteria; Acclimatization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000500368
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Occurrence of diazotrophic bacteria in Araucaria angustifolia Scientia Agricola
Neroni,Rafaela de Fátima; Cardoso,Elke Jurandy Bran Nogueira.
Araucaria angustifolia is an environmentally threatened tree and the whole biota of the Araucaria Forest should be investigated with the aim of its preservation. Diazotrophic bacteria are extremely important for the maintenance of ecosystems, but they have never been studied in Araucaria Forests. In this study, diazotrophic bacteria were isolated from Araucaria roots and soil, when grown in semi-specific, semi-solid media. The diazotrophic character of some recovered isolates could be confirmed using the acetylene reduction assay. According to their 16S rRNA sequences, most of these isolates belong to the genus Burkholderia.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Biological nitrogen fixation; Endophytic bacteria; Rhizosphere; Forest.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000300015
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Endophytic interaction of Bacillus sp. in micropropagated banana plantlets Anais da ABC (AABC)
ROCHA,JOSIELE S.; NIETSCHE,SILVIA; PEREIRA,MARLON C.T.; MARIA O.,MERCADANTE-SIMÕES; SANTOS,RAYANE C.; XAVIER,ADELICA A..
Abstract: The banana tree is associated with different species of endophytic bacteria that can stimulate plant growth. However, further studies are needed to better understand the relationships between this group of bacteria and the host plant. The objective of this study was to investigate the localization of the EB-40 (Bacillus sp.) through anatomical and ultrastructural analyses in micropropagated banana plantlets. The results demonstrated the effective colonization of the EB-40 isolate in the intercellular and intracellular spaces, as well as in the rhizosphere region. The wall of endophytic bacteria contains calcium and nitrogen. The EB-40 isolate was also observed to associate with the plasma membrane and cell wall. These results further our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Musa spp.; Light microscopy; Transmission electron microscopy..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500855
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Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Carobinha-do-campo) BABT
Carrim,Aysha Jussara Ivonilde; Barbosa,Edweis Cândida; Vieira,José Daniel Gonçalves.
The objectives of this work was to isolate endophytic bacteria from Jacaranda decurrens Cham. and screening of some enzymes of biotechnological interest. Ten (10) bacterial species were isolated and identified from the leaves and steams. All the isolates presented enzymatic activity, which was ranked as follows: proteolytic (60%) and amilolytic activity (60%), lipolytic (40%), esterasic (40%). However, cellulolytic and pectinolytic activities were not detected. This is the first report on the isolation and identification of endophytic bacteria from Jacaranda decurrens Cham.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Jacaranda decurrens; Enzymes.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400001
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Endophytic Microbiota Associated with the Root Tips and Leaves of Baccharis dracunculifolia BABT
Santana,Raiana S. M.; Fernandes,G. W.; Ávila,Marcelo P.; Reis,Mariana P.; Araújo,Flávio M. G. de; Salim,Anna C. M.; Oliveira,Guilherme; Chartone-Souza,Edmar; Nascimento,Andréa M. A..
ABSTRACT Although endophytic bacteria impact the health, and ultimately the fitness, of their hosts, our understanding of the diversity of endophytic species remains limited. Here we report on the endophytic microbiota inhabiting the roots, healthy leaves and leaves attacked by a gall-inducing insect of Baccharis dracunculifolia, a species of major economic relevance in South America, using 16S rRNA gene new generation sequencing. Rhodoplanes and Nitrospira were well represented in the communities of roots and leaves; known to be important for nitrogen cycling. The difference in bacterial diversity between healthy and galled leaves was not pronounced. The leaves seem to harbor specialized bacteria with high tolerances to abiotic stresses such as wide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Baccharis dracunculifolia; Endophytic bacteria; 16S rRNA.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100433
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Diversity of Endophytic and Epiphytic Bacteria From Sugarcane in Khuzestan, Iran BABT
Rezamahalleh,Hossein Moazzen; Khodakaramian,Gholam; Hassanzadeh,Nader.
Abstract Diverse microorganisms are living as endophytes in plant tissues and as epiphytes on plant surfaces in nature. Commercial formulations of bacteria antagonist to plant pathogenic microbes and ice nucleation active bacteria have been utilized as an environmentally safe method to manage plant disease and to prevent frost damage respectively. Bacteria were isolated from the leaf and sheath of sugarcane (CP69-1026 CP57-614, CP48-103, CP73-21, and CP70-1143 cultivars) verities grown in the field in Khuzestan province, Iran. Bacteria were found in both sheaths and leaves of sugarcane plants which they were significantly higher in density in leaves and which most were endophytic. The bacterial strains were 10 groups on the basis of the biochemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rRNA; Diversity; Endophytic bacteria; Sugarcane; Khuzestan.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100429
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Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata BABT
Das,Gitishree; Patra,Jayanta Kumar; Choi,Jaehyuk; Baek,Kwang-Hyun.
ABSTRACT Equisetum arvense, a fern species possesses a number of pharmaceutical prospective. In the present study, a total of 103 endophytic bacteria isolated from E. arvense and were evaluated for their anticandidal property against five Candida species, two C. albicans, C. glabrata, C. saitoana and C. geochares. Out of them fifty one were identified as per the morphological and molecular characterisation using 16S rRNA gene sequencing and among them, ten promising endophytic bacteria were mentioned in the present study. Among ten endophytic bacteria, Psychrobacillus insolitus and Curtobacterium oceanosedimentum exerted highest anticandidal effect against C. albicans KACC 30062 and C. glabrata KBNO6P00368, with diameter of inhibition zones of 21.30±0.41...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticandidal effect; Endophytic bacteria; Equisetum arvense; Candida albicans; Candida glabrata.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100455
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Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis BJM
Salles,Joana Falcão; Gitahy,Patrícia de Medeiros; Skøt,Leif; Baldani,José Ivo.
The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Endophytic bacteria; Diazotrophic; Cry gene.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001
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Survival of endophytic diazotrophic bacteria in soil under different moisture levels BJM
Oliveira,André L.M.; Canuto,Erineudo L.; Silva,Edmilson E.; Reis,Veronica M.; Baldani,José I..
The effects of soil moisture on the survival of three diazotrophic bacteria species (Azospirillum amazonense, Gluconacetobacter diazotrophicus and Azospirillum brasilense) were tested. Soil moisture had little influence on the survival of A. brasilense, which is considered a free-living species. On the other hand, increased soil moisture extended the survival of the endophytes A. amazonense and G. diazotrophicus. These results indicate that nitrogen-fixing endophytic species are more affected by soil moisture than associative nitrogen-fixing species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Soil survival; Gluconacetobacter diazotrophicus; Azospirillum amazonense; Azospirillum brasilense.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300005
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Illumina-based analysis of endophytic bacterial diversity of tree peony (Paeonia Sect. Moutan) roots and leaves BJM
Yang,Ruixian; Liu,Ping; Ye,Wenyu.
ABSTRACT Diverse communities of bacteria inhabit plant tissues and those bacteria play a crucial role for plant health and growth. Tree peony (Paeonia Sect. Moutan) is known for its excellent ornamental and medicinal values as Chinese traditional plant, but little is known about its associated bacterial community under natural conditions. To examine how endophytic bacteria in tree peony vary across tissues and cultivars, PCR-based Illumina was applied to reveal the diversity of endophytic bacteria in tree peony. A total of 149,842 sequences and 21,463 operational taxonomic units (OTUs) were obtained. The OTU abundance of roots was higher than leaves across other three cultivars except for ‘Kinkaku’ and ‘Luoyanghong’. The community was composed of five...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paeonia Sect; Moutan; Endophytic bacteria; Biodiversity; Illumina Miseq.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400695
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Mangrove endophyte promotes reforestation tree (Acacia polyphylla) growth BJM
Castro,Renata Assis; Dourado,Manuella Nóbrega; Almeida,Jaqueline Raquel de; Lacava,Paulo Teixeira; Nave,André; Melo,Itamar Soares de; Azevedo,João Lucio de; Quecine,Maria Carolina.
ABSTRACT Mangroves are ecosystems located in the transition zone between land and sea that serve as a potential source of biotechnological resources. Brazil's extensive coast contains one of the largest mangrove forests in the world (encompassing an area of 25,000 km2 along all the coast). Endophytic bacteria were isolated from the following three plant species: Rhizophora mangle, Laguncularia racemosa and Avicennia nitida. A large number of these isolates, 115 in total, were evaluated for their ability to fix nitrogen and solubilize phosphorous. Bacteria that tested positive for both of these tests were examined further to determine their level of indole acetic acid production. Two strains with high indole acetic acid production were selected for use as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; IAA; Phosphorus and plant growth promoting bacteria (PGPB).
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100059
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Isolation and characterization of endophytic bacteria isolated from the leaves of the common bean (Phaseolus vulgaris) BJM
Costa,Leonardo Emanuel de Oliveira; Queiroz,Marisa Vieira de; Borges,Arnaldo Chaer; Moraes,Celia Alencar de; Araújo,Elza Fernandes de.
The common bean is one of the most important legumes in the human diet, but little is known about the endophytic bacteria associated with the leaves of this plant. The objective of this study was to characterize the culturable endophytic bacteria of common bean (Phaseolus vulgaris. leaves from three different cultivars (Vermelhinho, Talismã, and Ouro Negro) grown under the same field conditions. The density of endophytic populations varied from 4.5 x 10² to 2.8 x 10³ CFU g-1 of fresh weight. Of the 158 total isolates, 36.7% belonged to the Proteobacteria, 32.9% to Firmicutes, 29.7% to Actinobacteria, and 0.6% to Bacteroidetes. The three P. vulgaris cultivars showed class distribution differences among Actinobacteria, Alphaproteobacteria and Bacilli. Based...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Common bean; Phaseolus vulgaris; 16S rDNA; Diversity indices.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400041
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Identification and characterization of the endophytic plant growth prompter Bacillus Cereus strain mq23 isolated from Sophora Alopecuroides root nodules BJM
Zhao,Longfei; Xu,Yajun; Sun,Ran; Deng,Zhenshan; Yang,Wenquan; Wei,Gehong.
Endophytes MQ23 and MQ23R isolated from Sophora alopecuroides root nodules were characterized by observing their ability to promote plant growth and employing molecular analysis techniques. Results showed that MQ23 and MQ23R are potential N2-fixing endophytes and belong to the same species as Bacillus cereus. MQ23 was shown to be able to produce siderophores, IAA, and demonstrate certain antifungal activity to plant pathogenic fungi. Co-inoculation with MQ23+MQ23II showed a more significant effect than inoculation alone in vitro for most of positive actions suggesting they have a cooperative interaction. Results of plant inoculation with endophytes indicated that the growth indexes of co-inoculated MQ23+MQ23II were higher than those of inoculated alone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth promoting characterization; Sophora alopecuroides; Root nodules; Endophytic bacteria; Bacillus cereus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200022
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Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.) BJM
Figueiredo,José Edson Fontes; Gomes,Eliane Aparecida; Guimarães,Claudia Teixeira; Lana,Ubiraci Gomes de Paula; Teixeira,Marta Aparecida; Lima,Guilherme Vitor Corrêa; Bressan,Wellington.
Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Bacillus; Sweet corn; SDS-PAGE; RDNA sequencing.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300014
Registros recuperados: 21
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