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| Valente,Angela M.M.P.; Ferreira,Antonio G.; Daolio,Cristina; Rodrigues Filho,Edson; Boffo,Elisangela F.; Souza,Antonia Q.L.; Sebastianes,Fernanda L.S.; Melo,Itamar S.. |
| The chemical reactions carried out by microorganisms have been used as a tool in modern chemistry. This paper reports the production of mycophenolic acid and a new phthalide by the endophytic fungus Penicillium crustosum obtained from coffee seeds. The fungus was cultivated in a liquid medium for a period of seven days and after that the culture medium was divided into four treatments: A, B, C and D, to which different organic substances were added. Treatment A was maintained as the control to evaluate the occurrence of biotransformation. Organic acids were added to the culture media of treatments B (ferulic and quinic acids) and C [cinnamic and 3,4-(methylenedioxy) cinnamic acids], and caffeine was added in the treatment D. All these organic compounds... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffee; Endophytic fungus; Mycophenolic acid; Penicillium crustosum; 5-hydroxy-7-methoxy-4-methylphthalide. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200487 |
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| Shentu,Xuping; Zhan,Xiaohuan; Ma,Zheng; Yu,Xiaoping; Zhang,Chuanxi. |
| The endophytic fungus strain 0248, isolated from garlic, was identified as Trichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8SITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13-epoxy-Δ9-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 µgmL-1. Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 µgmL-1. However, a relatively poor inhibitory effect was observed for trichodermin against Colletotrichum lindemuthianum (EC50 = 25.60 µgmL-1).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichoderma brevicompactum; Garlic; Endophytic fungus; Trichodermin; Antifungal activity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100036 |
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| Chaichanan,Jirapan; Wiyakrutta,Suthep; Pongtharangkul,Thunyarat; Isarangkul,Duangnate; Meevootisom,Vithaya. |
| To optimize the medium for high zofimarin production, sucrose maltose, glucose, tryptone and peptone were used in an orthogonal array design experiment, where the highest value of zofimarin produced was 25.6 µg/mL. This value was about 3 times higher than that obtained with Czapek yeast extract (CzYE) culture medium. A study with Plackett-Burman design showed that sucrose, maltose, glucose and NaNO3 were significant factors in zofimarin production. Further studies using central composite design (CCD) showed the significance of glucose and the interactions of these critical components affecting zofimarin production. Multiple regression analysis of the data yielded a poor fit as shown by the mismatch of the model with these variable factors. When a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zofimarin; Secondary metabolite; Medium optimization; Statistical methods; Endophytic fungus. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100042 |
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| Zhao,Jianglin; Wang,Xiaohan; Sun,Weibo; Mou,Yan; Peng,Youliang; Zhou,Ligang. |
| Background: Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, was a high producer of palmarumycin C13 with various bioactivities. In the present study, the experimental designs based on statistics were employed to evaluate and optimize the medium for palmarumycin C13 production in mycelia liquid culture of Berkleasmium sp. Dzf12. Results: Among various carbon and nitrogen sources, glucose, peptone and yeast extract were found to be the most favourable for palmarumycin C13 production based on the one-factor-at-a-time experiments. After Plackett-Burman test on the medium, glucose, peptone and yeast extract were further verified to be the most significant factors to stimulate palmarumycin C13 accumulation. These three factors (i.e.,... |
| Tipo: Journal article |
Palavras-chave: Berkleasmium sp. Dzf12; Central composite design; Endophytic fungus; Mycelia liquid culture; Palmarumycin C13; Plackett-Burman test; Response surface methodology. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600016 |
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| Li,Peiqin; Luo,Haiyu; Meng,Jiajia; Sun,Weibo; Wang,Xiaohan; Lu,Shiqiong; Peng,Youliang; Zhou,Ligang. |
| Background Three oligosaccharides (EOS, WOS and SOS) were respectively prepared from the corresponding polysaccharides, namely exopolysaccharide (EPS), water-extracted mycelial polysaccharide (WPS) and sodium hydroxide-extracted mycelial polysaccharides (SPS) from the endophytic fungus Fusarium oxysporum Dzf17. In this study, the effects of EOS, WOS and SOS on the activities of the defense-related enzymes, namely phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD) in its host plant Dioscorea zingiberensis cultures were investigated. Results For the suspension cell cultures of D. zingiberensis, the highest PAL activity was induced by 0.5 mg/mL of WOS at 48 h after treatment, which was 4.55-fold as that of control. Both PPO and... |
| Tipo: Journal article |
Palavras-chave: Endophytic fungus; Phenylalanine ammonia lyase; Peroxidase; Polyphenoloxidase; Polysaccharide. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400003 |
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