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Density and diversity of diazotrophic bacteria isolated from Amazonian soils using N-free semi-solid media Scientia Agricola
Silva,Krisle da; Nóbrega,Rafaela Simão Abrahão; Lima,Adriana Silva; Barberi,Alexandre; Moreira,Fatima Maria de Souza.
Non-symbiotic diazotrophic bacteria are amongst the most important functional groups of soil-dwelling microorganisms. These bacteria contribute to plant growth predominantly through biological N2 fixation. Here, we evaluated the density and diversity of non-symbiotic diazotrophic bacteria in soils taken from diverse land use systems (LUS) in Amazonia using nitrogen-free media. A total of 30 soil samples were collected from the following LUS: pristine forest, young secondary forest, old secondary forest, agroforestry, agriculture and pasture. Bacterial density was evaluated by the most probable number (MPN) method utilizing N-free semi-solid media with varied compositions (JNFb, NFb, LGI and Fam). Individual isolates were characterized by colony and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Burkholderia sp.; Enterobacter sp.; Serratia sp.; Bacillus sp.; Klebsiella sp..
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000500002
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Isolation and characterization of arsenic resistant bacteria from wastewater BJM
Abbas,Syed Zaghum; Riaz,Mehwish; Ramzan,Naseem; Zahid,M. Tariq; Shakoori,Farah R.; Rafatullah,Mohd..
The present study proposed the isolation of arsenic resistant bacteria from wastewater. Only three bacterial isolates (MNZ1, MNZ4 and MNZ6) were able to grow in high concentrations of arsenic. The minimum inhibitory concentrations of arsenic against MNZ1, MNZ4 and MNZ6 were 300 mg/L, 300 mg/L and 370 mg/L respectively. The isolated strains showed maximum growth at 37 ºC and at 7.0 pH in control but in arsenite stress Luria Bertani broth the bacterial growth is lower than control. All strains were arsenite oxidizing. All strains were biochemically characterized and ribotyping (16S rRNA) was done for the purpose of identification which confirmed that MNZ1 was homologous to Enterobacter sp. while MNZ4 and MNZ6 showed their maximum homology with Klebsiella...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arsenite oxidizing bacteria; Bioremediation; Enterobacter sp.; Klebsiella pneumoniae.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400022
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