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Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats BJMBR
Liberatore,A.M.A.; Moreira,F.C.; Gomes,T.A.T.; Menchaca-Diaz,J.L.; Koh,I.H.J..
Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli (EPEC); Atypical EPEC; Bacterial translocation; Microcirculation; Tissue perfusion.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000007
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Virulence profiles of Shiga toxin-producing Escherichia coli and other potentially diarrheagenic E.coli of bovine origin, in Mendoza, Argentina BJM
Pizarro,M.A.; Orozco,J.H.; Degarbo,S.M.; Calderón,A.E.; Nardello,A.L.; Laciar,A.; Rüttler,M.E..
This study described a group of strains obtained from a slaughter house in Mendoza, in terms of their pathogenic factors, serotype, antibiotype and molecular profile. Ninety one rectal swabs and one hundred eight plating samples taken from carcasses of healthy cattle intended for meat consumption were analyzed. Both the swab and the plate samples were processed to analyze the samples for the presence of virulence genes by PCR: stx1, stx2, eae and astA. The Stx positive strains were confirmed by citotoxicity assay in Vero cells. The isolates were subsequently investigated for their O:H serotype, antimicrobial susceptibility and molecular profile by Random Amplification of Polymorphic DNA (RAPD). Twelve E.coli strains were identified by their pathogenicity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemolitic Uremic Syndrome (HUS); Shiga Toxin Escherichia coli (STEC); Enteroaggregative Stable toxin (EAST1); Enteropathogenic Escherichia coli (EPEC); Foodborne diseases; Cattle.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400021
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Distribution of non-LEE-encoded type 3 secretion system dependent effectors in enteropathogenic Escherichia coli BJM
Salvador,Fábia A.; Hernandes,Rodrigo T.; Vieira,Mônica A.M.; Rockstroh,Anna C.; Gomes,Tânia A.T..
Enteropathogenic Escherichia coli (EPEC) are important human gastroenteritis agents. The prevalence of six non-LEE genes encoding type 3 translocated effectors was investigated. The nleC, cif and nleB genes were more prevalent in typical than in atypical EPEC, although a higher diversity of genes combinations was observed in atypical EPEC.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli (EPEC); Type 3 secretion system; Pathogenicity; Diarrhea.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300014
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