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Registros recuperados: 78
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Internal quality of laying hen eggs fed on protease at different storage and stocking conditions Animal Sciences
Suckeveris, Diana; Muñoz, Julian Andres; Demuner, Leandro Félix; Caetano, Vinícius Camargo; Faria Filho, Daniel Emydgio de; Faria, Douglas Emygdio de.
Effects on diets with reduction of crude protein and methionine + cystine (RCP) and protease supplementation (PRO) for laying hens at different ages and their influence on the internal quality of eggs at different conditions (STO) and storage times (TM) are evaluated. Four hundred and twenty eggs from commercial Hy-Line W36 laying hens collected at 32, 44 and 58 weeks of age were used. Experimental design was completely randomized in a factorial scheme 3 x 2 x 2 x 2: RCP (control, enhancement of one and two times the enzyme protease), PRO (with and without protease), STO (room temperature and refrigerated) and TM (14 and 28 days), with seven replications of one egg per experimental unit. The factorial scheme 3 x 2 x 2 was used at 58 weeks of age, because...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.00.00-2; 5.04.03.00-1; 5.04.03.01-0 egg yolk; Egg white; Enzyme; Haugh unit; Nutrition; Poultry..
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/27929
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Effect of different levels of supplementary alpha-amylase in finishing broilers Animal Sciences
Castro, Solange de Faria; Bertechini, Antonio Gilberto; Lima, Eduardo Machado Costa; Clemente, Alisson Hélio Sampaio; Ferreira, Verônica Gabriela Gonçalves; Carvalho, Julio Cesar Carrera de.
The objective of this study was to investigate the effects of alpha-amylase supplementation on performance and carcass and cuts yield in broilers during finishing phase (21 to 42 days). A completely randomized experimental design with six treatments and eight replications was used: Positive Control (PC): no enzyme; Negative control (NC): without enzyme formulated with 6.34% less energy (- 6.34% ME); NC1: with 250 g ton-1 of alpha-amylase; NC2: with 500 g ton-1 of alpha-amylase; NC3: with 750 g ton-1 of alpha-amylase; NC4: with 1000 g ton-1 of alpha-amylase. Significant differences in weight gain, feed intake and feed conversion were reported with the inclusion of alpha-amylase. The NC treatment with inclusion of amylase provided improvement values of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Energy reduction; Enzyme; Performance.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/47546
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Effect of phytase on digestibility of corn, sorghum and wheat bran by silver catfish (Rhamdia voulezi) Animal Sciences
Signor, Altevir; Lewandowski, Vanessa; da Silva, Rodrigo Aguiar; Fries, Edionei Maico; Schuller, Jean Marcel.
This study aimed to evaluate the digestibility of plant energetic ingredients, corn, sorghum and wheat bran, with and without phytase supplementation, in silver catfish (Rhamdia voulezi). The experimental design was completely randomized, represented by eight treatments, which consisted of the development of a reference diet and a test diet for each ingredient evaluated (corn, sorghum and wheat bran), with and without supplemental phytase. Diets were formulated so as to contain 70% reference feed and 30% test ingredient. The digestibility coefficients of dry matter and crude protein were significantly influenced only in relation to the evaluated ingredients. Phytase influenced the digestibility coefficients of energy and phosphorus, with interaction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feeding; Enzyme; Plant energetic ingredients; Silver catfish.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/32054
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Effect of enzyme addition on energy utilization and performance of broiler chickens fed wheat-based diet with different metabolizable energy levels Animal Sciences
Moftakharzadeh, Seyed adel; Janmohammadi, Hossein; Taghizadeh, Akbar; Kianfar, Ruhollah; Olyayee, Majid Geshlog.
An experiment was carried out to investigate the effect of multi-enzyme in high and low levels of metabolizable energy (13.81 and 11.51 MJ kg-1 diet) on performance and energy utilization of broilers fed wheat-soybean meal diets from 0 to 21 days of age. Result showed that birds fed diets containing 11.51 MJ kg-1 consumed significantly (p < 0.05) more feed than diets containing 13.81 MJ kg-1, whereas daily gain and feed conversion ratio improved (p < 0.05) when enzyme was added to 11.51 MJ kg-1 diet. There was significant improvement in metabolizable energy, net energy for production, organic and dry matter digestibility in 0-21 when diets supplemented with enzyme (p < 0.05). Addition of enzyme to 11.51 MJ kg-1 containing diet significantly (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme; Net energy; Wheat; Body composition; Broilers.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/44585
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Influence of xylanase and vitamin A in wheat-based diet on performance, nutrients digestibility, small intestinal morphology and digesta viscosity in broiler chickens Animal Sciences
Khoramabadi, Vahid; Akbari, Mohammad Reza; Khajali, Fariborz; Noorani, Hossein; Rahmatnejad, Enayat.
The effect of wheat-based diet (WBD), supplemented with xylanase and surplus vitamin A, on performance, nutrient digestibility, intestinal morphology and digesta viscosity of broiler chickens was investigated. Based on a completely randomized design, 240-day-old chickens were randomly divided into six experimental diets and four replications with 10 chickens each. Experimental diets consisted of corn-based diet (CBD) (T1), WBD with routine amounts of vitamin A (9000 IU kg-1) (T2), T2 without vitamin A in premix (T3), T2 + 6000 IU kg-1 vitamin A (T4), T2 + 420 IU kg-1 xylanase (T5), and T2 + 6000 IU kg-1 vitamin A + 420 IU kg-1 xylanase (T6). For Feed Conversion Rate (FCR) between day 1 and 21 and between day 21 and 42 WBD supplemented with vitamin A and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Enzyme; Morphology; Viscosity; Vitamin A; Wheat.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/23910
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Optimization of asparaginase production from Zymomonas mobilis by continuous fermentation Biological Sciences
Menegat, Francieli Bortoluzzi; Baldo, Cristiani; Celligoi, Maria Antonia Pedrine Colabone; Buzato, João Batista.
Asparaginase is an enzyme used in clinical treatments as a chemotherapeutic agent and in food technology to prevent acrylamide formation in fried and baked foods. Asparaginase is industrially produced by microorganisms, mainly gram-negative bacteria. Zymomonas mobilis is a Gram-negative bacterium that utilizes glucose, fructose and sucrose as carbon source and has been known for its efficiency in producing ethanol, sorbitol, levan, gluconic acid and has recently aroused interest for asparaginase production. Current assay optimizes the production of Z. mobilis asparaginase by continuous fermentation using response surface experimental design and methodology. The studied variables comprised sucrose, yeast extract and asparagine. Optimized condition obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose; Asparagine; Yeast extract; Enzyme; Microbial production; Factorial design.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31057
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OPTIMIZATION OF THE CULTURE CONDITIONS FOR TANNASE PRODUCTION BY Aspergillus sp. GM4 UNDER SOLID-STATE FERMENTATION Biological Sciences
Souza, Patrícia Nirlane da Costa; Maia, Natália da Costa; Guimarães, Luís Henrique Souza; Resende, Mário Lúcio Vilela de; Cardoso, Patrícia Gomes.
The production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF)  was investigated using different vegetables leaves such as mango, jamun, coffee and agricultural residues such as coffee husks, rice husks and wheat bran. Among substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. The best incubation time was studied and others variables were optimized using the Central Composite Design. The best conditions for tannase production were: incubation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Surface response methodology; Enzyme; Jamun; Agricultural residues.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22731
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Dhal Recovery From Enzyme Pretreated Pigeon Pea Cultivar Gjp1 CIGR Journal
Dabhi, Mukesh N.; Sangani, V P; Rathod, P J.
GJP1 variety is newly developed variety of pigeon pea. It gives higher seed yield as compared to other varieties. Pigeon pea is mostly consumed in the form of splits hence, it is necessary to verify its hulling efficiency and utmost dhal recovery. Pre milling treatments are generally employed to loosen the seed coat to remove husk without losing any edible portion and better dhal recovery.  Xylanase, pectinase and cellulose enzymes were used to evaluate the milling properties of pigeon pea grains. Efforts were made to evaluate the effect of enzymatic parameters, i.e., enzyme concentration (20-50 mg/100g dry matter), incubation time (4-12 h), incubation temperature (35-55 oC) and tempering water pH (4.0-6.0) on hulling efficiency were optimized using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Processing; Milling; Enzyme Pigeon pea; Enzyme; Dhal recovery; Pretreatment; Hulling efficiency.
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4762
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Expression of manganese peroxidase by Lentinula edodes and Lentinula boryana in solid state and submerged system fermentation Anais da ABC (AABC)
HERMANN,KATIA L.; COSTA,ALESSANDRA; HELM,CRISTIANE V.; LIMA,EDSON A. DE; TAVARES,LORENA B.B..
The production of ethanol from lignocellulosic biomass is referred as a second generation biofuel, whose processing is one of the most promising technologies under development. There are few available studies on the use of enzymes produced by fungi as active for the biodegradation of lignocellulosic biomass. However, the manganese peroxidase (MnP) enzyme presents high potential to degrade lignin and the basidiomycetes are the major producers of this oxidase. Thus, this study aimed at evaluating the ability of fungi Lentinula edodes and Lentinula boryana to produce this enzyme when cultivated in submerged fermentation system (SS) and also in solid-state fermentation system (SSF) containing Eucalyptus benthamii sawdust with or without corn cob meal. In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Basidiomycetes; Eucalyptus benthamii; Enzyme; Ethanol.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300965
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Inactivation of yeast inorganic pyrophosphatase by organic solvents Anais da ABC (AABC)
Grazinoli-Garrido,Rodrigo; Sola-Penna,Mauro.
A number of application for enzymes in organic solvents have been developed in chemical processing, food related conversions and analyses. The only unsolved problem related to nonaqueous enzymology is the notion that enzymes in organic solvent are mostly far less active than in water. Therefore, studies concerning the mechanisms by which enzymes are inactivated by organic solvents would reveal a clear understanding of the structure-function relationship of this phenomenon. Here we analyzed the effects of a series of alcohols (methanol, ethanol, 1-propanol and 2-propanol) and acetone on the activity of yeast inorganic pyrophosphatase. We observed that solvents inactivated the enzyme in a dose-dependent manner. This inactivation is also dependent on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Organic solvents; Enzyme; Yeast inorganic pyrophosphatase; Alcohol.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400006
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Growth performance and carcass yield of broiler chickens in response to carbohydrases and its association with phytase Arq. Bras. Med. Vet. Zootec.
Dessimoni,G.V.; Sakomura,N.K.; Donato,D.C.Z.; Soares,L.; Sarcinelli,M.F.; Malheiros,E.B.; Dalólio,F.S..
ABSTRACT An experiment was conducted to evaluate broiler chicken performance and carcass yield in response to carbohydrase supplementation (Xylanase and Betaglucanase; XB) and association of carbohydrase and phytase (PHY). A total of 1,920 day-old male broiler chicks (Cobb 500) were distributed in a completely randomized design with 8 treatments and 8 replicates each, allocated in 64 pens with 30 chicks each. The treatments were: Positive Control (PC); Negative Control (NC): reduced ME [-120kcal/kg (Starter) and -170kcal/kg (Finisher)]; NC + XB [50g/ton]; NC + XB [100g/ton]; NC + XB [150g/ton]; NC + XB [50g/ton] + PHY [100g/ton]; NC + XB [100g/ton] + PHY [100g/ton]; NC + XB [150g/ton] + PHY [100g/ton]. The inclusion of XB (150) and XB (50, 100, and 150)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additives; Corn; Metabolizable energy; Enzyme; Soybean meal.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300983
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Purification and characterisation of trypsin-like enzyme from the pyloric caeca of cod (Gadus morhua) II BABT
Beirão,Luiz Henrique; Mackie,Ian Mckintoch; Teixeira,Evanilda; Damian,César.
A trypsin -like enzyme from the pyloric caeca of cod (Gadus morhua) was purified by affinity chromatography on CHOM Sepharose 4B. Some characteristics were established by its catalytic activity on T.A.M.E., typical enzyme substrate, and serine protease inhibitors. The enzyme had an isoelectric point of 5.30 and 5.89 and was very similar in amino acid composition to bovine trypsin, but differed in having a higher relative amount of acidic amino acids and a lower amount of basic amino acids. The enzyme also hydrolysed fish protein substrates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cod; Enzyme; Trypsin.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100005
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Production of tannase by Aspergillus tamarii in submerged cultures BABT
Costa,Andréa M.; Ribeiro,Wanessa X.; Kato,Elaine; Monteiro,Antonio Roberto G.; Peralta,Rosane Marina.
The production of tannase by Aspergillus tamarii was studied in submerged cultures. The fungus produced an extracellular tannase after two days of growth in mineral medium containing tannic acid, gallic acid and methyl gallate as carbon source. The best result was obtained using gallic acid as inducer (20.6 U/ml). The production of enzyme was strongly repressed by the presence of glucose. Crude enzyme was optimally active at pH 5.0 and 30º C. The enzyme was stable in a large range of pH and up to the temperature of 45º C.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus tamarii; Enzyme; Fungal biomass; Submerged cultures; Tannase; Tannins.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200021
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Use of vinasse and sugarcane bagasse for the production of enzymes by lignocellulolytic fungi BABT
Aguiar,Mario Mamede; Ferreira,Luiz Fernando Romanholo; Monteiro,Regina Teresa Rosim.
In this present work, three strains of Pleurotus and Trichoderma reesei were cultivated in media with pre-treated bagasse and vinasse. Cellulolytic and lignolytic activities and biomass production were analyzed. The treatment of the bagasse with 2% H2O2 + 1.5% NaOH + autoclave resulted in a greater fiber breakage increasing the cellulose level up to 1.2 times and decreasing 8.5 times the hemicellulose content. This treatment also resulted in a high lignolytic activity for all cultures utilized. T. reesei produced laccase, peroxidase and manganese-peroxidase in all the treatments, having its manganese-peroxidase activity raging from 1.9 to 4.8 times higher than the basidiomycetes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bagasse; Enzyme; Fungi; Lignocellulolytic; Pleurotus; Sugarcane.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500030
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Chromatographic characterization of products isolated from chrome shavings BABT
Garcia,Edivaldo E; Kimura,Cláudia C.M; Martins,Ariovaldo C; Rocha,Gisele O; Nozaki,Jorge.
Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chrome shavings; Enzyme; Chromatography; Protein; Chromium.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300003
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Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed BABT
Das,Mithu; Banerjee,Rintu; Bal,Satish.
The aim of this study was to evaluate the interaction effects of the physico-chemical parameters on the endoglucanase (CMCase) production by Trichoderma reesei Rut C30 on a cellulosic agro-residue by the solid-state fermentation (SSF) and to determine their optimum values by the EVOP factorial design technique. The best combination of physical parameters for the maximum production of the endoglucanase (CMCase) was 28ºC temperature, 79% relative humidity and 4.8 pH of the medium. The best combination of the chemical parameters was (mg/L) nicotinic acid 15, naphthalene acetic acid 7, ferric chloride 5 and Tween-80 6. With the application of this technique, the yield of the CMCase increased by ~ 2.3 fold.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme; Endoglucanase; Carboxymethylcellulase (CMCase); EVOP factorial design technique.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100005
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Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum BABT
Gasparotto,Elainy Patrícia Lino; Abrão,Sorrailla Carolina Carrard; Inagaki,Suely Yaeko; Tessmann,Dauri José; Kemmelmeier,Carlos; Tessmann,Ione Parra Barbosa.
A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Galactose oxidase; Fusarium graminearum; Enzyme.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500004
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Comparisons Between Different Methods in Measuring Enzyme Similarity for Metabolic Network Alignment BABT
Zhou,Wenwei; Liu,Jiangtao; Xing,Hiu; Zhang,Zhengdong; Xie,Xiaoyao; Jing,Fengxuan.
Metabolic network alignments enable comparison of the similarities and differences between pathways in two metabolic networks and help to uncover the conserved sub-blocks therein. Such analysis is important in the understanding of metabolic networks and species evolution. The fundamental parts of metabolic network alignment algorithms all involve comparisons of the similarity between two enzymes as a similarity measure of network nodes. As a result, the study of methods for measuring enzyme similarity becomes highly relevant. Currently, two approaches are mainly used to measure enzyme similarity. One of the methods is based on similarity measures of gene or protein sequences; the other is based on enzyme classification. In this study, multiple metabolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolic network alignment; Enzyme; Similarity measure; Classification numbering; Sequence similarity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100405
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Co-production of Proteases and Bioactive Protein Hydrolysates from Bioprocessing of Feather Meal BABT
Bernardo,Bernardete da Silva; Ramos,Rodrigo Ferraz; Callegaro,Kelly; Daroit,Daniel Joner.
Abstract: Feather meal conversion through submerged cultivations with Bacillus strains (CL33A, CL14) yielded proteases and protein hydrolysates. After 4-day (CL33A) and 10-day (CL14) cultivations, protease activities reached 461 U/mL; hydrolysates presented antioxidant (radicals-scavenging, 57-77%; Fe2+-chelation, 14-28%; Fe3+-reduction) and antidiabetic (dipeptidyl peptidase-IV inhibition, 49-52%) potentials. The obtained bioproducts present prospective commercial/industrial applications.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Bioprocess; Enzyme; Antioxidant; Antidiabetic.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100220
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Acid and enzymatic hydrolysis to recover reducing sugars from cassava bagasse: an economic study BABT
Woiciechowski,Adenise Lorenci; Nitsche,Saul; Pandey,Ashok; Soccol,Carlos Ricardo.
The objective of this work was to study the acid and enzymatic hydrolysis of cassava bagasse for the recovery of reducing sugars and to establish the operational costs. A statistical program "Statistica", based on the surface response was used to optimize the recovery of reducing sugars in both the processes. The process economics was determined considering the values of reducing sugars obtained at laboratory scale, and the operations costs of a cylindrical reactor of 1500 L, with flat walls at the top and bottom. The reactor was operated with 150 kg of cassava bagasse and 1350 kg of water. The yield of the acid hydrolysis was 62.4 g of reducing sugars from 100 g of cassava bagasse containing 66% starch. It represented 94.5% of reducing sugar recovery. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cassava bagasse; Hydrolysis; Acid; Enzyme; Reducing sugars; Economics.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300018
Registros recuperados: 78
Primeira ... 1234 ... Última
 

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