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| Registros recuperados: 35 | |
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| Al-Motabagani,Mohamed Akram. |
| Methotrexate (MTX) is widely used in the therapy of various types of malignancy. The present work was designed to investigate the histological and histochemical changes in the liver of albino rat following methotrexate administration. Fifteen adult male albino rats were used in the present work. They were divided into three main groups: Group I was kept without treatment and served as control. Groups II and III were given intraperitoneal injections of normal saline and MTX, respectively, at a dosage of (0.5 mg/Kg) twice weekly for total durations of 3, 6 and 9 weeks. The rats were sacrificed and the livers were excised and processed for histological and histochemical study. Examination of sections of the livers of group III showed mononuclear cell... |
| Tipo: Journal article |
Palavras-chave: Methotrexate; Hepatocytes; Hepatic lobules; Lycogen; Enzyme activity. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400020 |
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| Yap,MKK; Tan,NH; Fung,SY. |
| The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks exhibit enzymatic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Naja; Cobra venom; Enzyme activity; Cardiotoxin. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400012 |
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| Bordon,Karla C. F.; Wiezel,Gisele A.; Cabral,Hamilton; Arantes,Eliane C.. |
| BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated through cation exchange, gel filtration and affinity chromatography, followed by a reversed-phase fast protein liquid chromatography to confirm its purity. Subsequently, its N-terminal amino acid sequence was determined by Edman degradation. The enzyme activity and stability were evaluated by a microplate colorimetric assay and the molecular mass was estimated by SDS-PAGE using periodic acid-Schiff... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; L-amino acid oxidase; Rattlesnake; Enzyme activity; Enzyme stability; Chromatography; Snake venom; Yellow venom; Stabilization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100335 |
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| Martínez-Cárdenas,Leonardo; Frías-Quintana,Carlos A.; Álvarez-González,Carlos A.; Jiménez-Martínez,Luis D.; Martínez-García,Rafael; Hernández-Almeida,Oscar U.; Bello-Pineda,Javier; Arellano-Méndez,Leonardo U.; Ponce-Palafox,Jesús T.. |
| ABSTRACT Short-tailed pipe fish (Microphis brachyurus) is a freshwater organism with high economic potential for the aquarium hobby, so it is necessary to implement methods to promote its culture through studies of digestive physiology. General activities of acid and alkaline proteases were evaluated, as well as the effect of pH, temperature and inhibitors. The optimal pH of stomach proteases was 2, while the optimal pH of intestinal proteases was 10. Optimal temperature for the acidic proteases was 35 ºC, while for alkaline proteases it was 45 ºC. Thermal stability showed high resistance at 35 ºC for both acid and alkaline proteases (above 100% residual activity). Acid proteases are resistant at pH 2 (50% of residual activity), meanwhile alkaline... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme activity; Sygnathidae; PH; Protease inhibitors; Temperature. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000200212 |
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| Godínez, S.; Calderón, M.. |
| Se realizó la evaluación de 10 cepas de hongos filamentosos pertenecientes a la especie Penicillium camemberti de utilidad en la elaboración de quesos Camembert y conservadas por liofilización entre 10 y 20 años en un banco de cepas. El estudio comprendió la determinación de porcentaje de retención de viabilidad, crecimiento en medio sólido, características macromorfológicas, características micromorfológicas, capacidad proteolítica y lipolítica. Los resultados mostraron que el empleo de este método aseguró una supervivencia de 100 % de las cepas, no detectándose alteraciones en sus características macromorfológicas y micromorfológicas. Los cultivos mantuvieron sus características de crecimiento y actividad enzimática. |
| Tipo: Journal Contribution |
Palavras-chave: Growth; Growth; Enzyme activity; Viability. |
| Ano: 2008 |
URL: http://hdl.handle.net/1834/4842 |
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| Godínez, S.; Calderón, M.. |
| Se realizó la evaluación de 10 cepas de hongos filamentosos pertenecientes a la especie Penicillium camemberti de utilidad en la elaboración de quesos Camembert y conservadas por liofilización entre 10 y 20 años en un banco de cepas. El estudio comprendió la determinación del porcentaje de retención de viabilidad, crecimiento en medio sólido, características macromorfológicas, características micromorfológicas, capacidad proteolítica y lipolítica. Los resultados mostraron que el empleo de este método aseguró una supervivencia de 100 % de las cepas, no detectándose alteraciones en sus características macromorfológicas y micromorfológicas. Los cultivos mantuvieron sus características de crecimiento y actividad enzimática. |
| Tipo: Journal Contribution |
Palavras-chave: Growth; Enzyme activity. |
| Ano: 2008 |
URL: http://hdl.handle.net/1834/4725 |
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| Abrameto1,MA; Pozzo Ardizzi,CM; Gil,MI; Molina,LM. |
| Costly investments in storage and transport of onion bulbs makes it increasingly important to identify cultivars with the best chance of long-term storage. This paper discusses our own and other researchers' results and laboratory procedures on onion's pungency, soluble solids, dry matter, respiration rate, carbohydrates and catabolism-related enzymes under different storage conditions. The variability in parameters such as carbohydrate composition, pungency, soluble solids and soluble uronic acid concentration in water is growth dependent, and defines the quality of onion bulbs at harvest time. During storage, these parameters can be modified by environmental conditions, presence of pathogens or termination of dormancy. Under postharvest conditions,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa L.; Respiration rate; Sprouting; Ethylene; Non-structural carbohydrates; Enzyme activity. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200003 |
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| Amaral,Lourdes Isabel Velho do; Santos,Henrique P.; Rossatto,Davi Rodrigo; Buckeridge,Marcos Silveira. |
| The cotyledons of Hymenaea courbaril store large amounts of xyloglucan, a cell wall polysaccharide that is believed to serve as storage for the period of seedling establishment. During storage mobilisation, xyloglucan seems to be degraded by a continuous process that starts right after radicle protrusion and follows up to the establishment of photosynthesis. Here we show evidence that events related to the hydrolases activities and production (α-xylosidase, β-galactosidase, β-glucosidase and xyloglucan endo-β-transglucosilase) as well as auxin, showed changes that follow the diurnal cycle. The period of higher hydrolases activities was between 6pm and 6am, which is out of phase with photosynthesis. Among the enzymes, α-xilosidase seems to be more important... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme activity; IAA; Α-xylosidase; XTH; Xyloglucan. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400008 |
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| Yaghobfar,A; Kalantar,M. |
| ABSTRACT The experiment was carried out in a completely randomized design (CRD) with 625 broiler chicks (Ross 308) for 5 repetitions (25 birds per each replicated) on the 5 treatments diet. Treatments included two different types of cereal grains (wheat, and barley) with or without an enzyme supplementation along with a corn-based diet as control group. The experimental diets were formulated to have similar contents of crude protein, metabolizable energy, total non-starch polysaccharides (NSP) and were fed in two periods of starter and grower. Experimental traits were consisted growth performance, ileal flora numeration, villus morphology in 3 parts of the intestine, digesta viscosity and pancreatic enzyme activity, and determining the gene expression... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broilers; Enzyme activity; Gene expression; Micro flora; NSP. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400629 |
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| Horvatovic,MP; Glamocic,D; Zikic,D; Hadnadjev,TD. |
| Enzyme supplementation of diets enhances broiler performance by improving some of the basic production parameters such as average feed intake, feed conversion ratio, or average weight gain. The enzyme NSPase is commonly used in broiler diets containing high levels of viscous cereals such as barley, oat, wheat, or sorghum. The use of NSPase in diets with different levels of sunflower meal has been not been extensively explored. The experiment was carried out to evaluate the effects of the inclusion of sunflower meal levels in grower and finisher broiler diets supplemented or not with enzymes (cellulase, β-glucanase, and xylanase) on broiler performance, intestinal function, and carcass traits. A completely randomized experimental design, with 3*2 factorial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carcass characteristics; Enzyme activity; Performance; Viscosity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000100025 |
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| Quintela,Matheus P.; Pedrosa,Elvira M. R.; Willadino,Lilia; Rolim,Mario M.; Silva,Ênio F. de F. e; David,Mariana F. de L.. |
| ABSTRACT This study aimed to evaluate the interaction between intensity and duration of water deficit (90, 56 and 22% of pot capacity [PC] for 30, 60 and 90 days under continuous stress) associated to the parasitism of the nematode Meloidogyne incognita on the growth of the sugarcane variety RB92579 and the activity of the enzymes catalase and ascorbate peroxidase. The experiment was conducted in completely randomized design in a 7 x 2 factorial scheme (seven water deficit treatments: control [90% PC], 56% PC for 30, 60 and 90 days, 20% PC for 30, 60 and 90 days; and two densities of M. incognita: 0 and 20000 eggs plant-1), with four replicates. The water stress corresponding to 56% PC for 30 or 60 days did not affect RB92579 development. The evaluated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme activity; Biomass; Root-knot nematode; Saccharum. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662015000600581 |
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| Silva,Patrícia Pereira da; Sekita,Marcelo Coelho; Dias,Denise Cunha Fernandes dos Santos; Nascimento,Warley Marcos. |
| ABSTRACT In carrot seed production, harvesting of the umbels is generally staggered, or sometimes the whole plant is harvested with the umbels at different stages of maturation; in that case, there is a risk of harvesting immature seeds and of losses due to natural dehiscent, or to physiological deterioration as a result of unfavourable environmental factors. The present study aimed to evaluate the physiological and biochemical changes related to the maturation of 'Planalto' carrot seeds from diferent umbels. The samples were collected 120 days after anthesis, with the primary, secondary and tertiary umbels being harvested separately. Seeds were analysed for germination, first count and germination rate, at temperatures of 25 ºC (optimum) to 35 ºC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Daucus carota L.; Physiological quality; Enzyme activity. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000200407 |
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| Silva,Andréa Scaramal da; Jacques,Rodrigo Josemar Seminoti; Andreazza,Robson; Bento,Fátima Menezes; Camargo,Flávio Anastácio de Oliveira. |
| Phenanthracene is a highly toxic organic compound capable of contaminating water and soils, and biodegradation is an important tool for remediating polluted environments. This study aimed to evaluate the effects of trace elements, cations, and environmental conditions on the activity of the protocatechol 3,4-dioxygenase (P3,4O) enzyme produced by the isolate Leifsonia sp. in cell-free and immobilized extracts. The isolate was grown in Luria Bertani broth medium (LB) amended with 250 mg L-1 of phenanthrene. Various levels of pH (4.0-9.0), temperature (5-80 °C), time (0-90 min), trace elements (Cu2+, Hg2+ and Fe3+), and cations (Mg2+, Mn2+, K+ and NH4+) were tested to determine which conditions optimized enzyme activity. In general, the immobilized extract... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme activity; Enzyme immobilization; Biodegradation; Waste treatment. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200002 |
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| Nascimento,Warley Marcos. |
| Ethylene can stimulate seed germination and overcome dormancy in many species. For instance, the inhibitory effect of high temperature on lettuce seed germination can be overcome by exogenous ethylene. Involvement of ethylene in seed germination is a widely accepted fact, but the mechanistic details are poorly understood. A critical factor in ethylene studies is the response of seed germination properties to various ethylene inhibitors. Lettuce seed has been used as a model to study the role of ethylene in seed germination at high temperature. The role of ethylene in germination is not known but either ethylene evolution or addition of 1-aminocyclopropane-1-carboxilic acid (ACC) have been associated with increased activity of endo-beta-mannanase, a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactuca sativa; Thermoinhibition; Dormancy; Enzyme activity. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000300029 |
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| Registros recuperados: 35 | |
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