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Use of RAPD, enzyme activity staining, and colony size to differentiate phytopathogenic Fuzarium Oxysporum isolates from Iran BJM
Motallebi,Mostafa; Zamani,Mohammad Reza; Jazayeri,Omid; Harighi,Mohammad Javad.
Fusarium oxysporum is a common soilborn plant pathogen with a worldwide distribution. Fusarium yellows disease of chickpea (Cicer arientinum) caused by F.oxysporum is one of the most destructive soilborn disease which is a major production constraint in chickpea-growing regions of Iran. Three laboratory methods "amplification of genomic DNA using random primers, enzyme activity staining, and colony size determination" have been used to discriminate between highly virulent (HV) and weakly virulent (WV) isolates of F. oxysporum. On the basis of colony size (a traditional morphological method) and the ability of isolates to produce pectic enzymes, five HV isolates were differentiated from three WV isolates. The HV isolates formed large colony (ranging from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium oxysporum; RAPD; Colony size; Enzyme activity staining.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400004
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