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Properties of the enzyme acetolactate synthase in herbicide resistant canola Bragantia
Durigon,Miria Rosa; Mariani,Franciele; Santos,Fernando Machado dos; Vargas,Leandro; Chavarria,Geraldo.
ABSTRACT Mutations in the gene that encodes the enzyme acetolactate synthase (ALS), conferring plant resistance to ALS inhibitor herbicides, can reduce or inhibit enzymatic function. The objective of this work was to determine the functionality and kinetic characteristics of the enzyme ALS and evaluate the action of the herbicides imazapic (imidazolinone) and metsulfuron-methyl (sulfonylurea) on the activity of the enzyme ALS in canola hybrid Hyola 571CL (imidazolinone resistant), compared to hybrids Hyola 555TT (triazine resistant) and Hyola 61 (conventional). The plants were grown in a greenhouse, and leaves were collected to perform ALS activity analysis. The concentration of pyruvate to provide initial velocity equal to half the maximum reaction rate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brassica napus L.; Enzyme functionality; Metsulfuron-methyl; Imazapic.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000300485
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