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	Provedor de dados BJMBR (1) BJPS (1) Autor Hongfang,W. (1) Jianmin,Z. (1) Meifu,F. (1) Singh,Anu (1) Talegaonkar,Sushama (1) Tariq,Mohammad (1) Thomas,Shindu (1) Mais... Palavra-chave Epirubicin (2) Drug resistance (1) Forced degradation (1) HPLC (1) Hepatocellular carcinoma (1) Microcarrier (1) Polymeric nanoparticles (1) Quality control (1) Spheroids (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells BJMBR Jianmin,Z.; Hongfang,W.; Meifu,F.. The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by scanning electron microscopy, transmission electron microscopy and flow cytometry, respectively. The IC50 was determined by flow cytometry and MTT assay after the cells cultured in aggregates and monolayers were treated with pharmorubicin. The culture products exhibited structural characteristics somewhat similar to those of trabecular hepatocarcinoma in vivo. Among the microcarriers, cells were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatocellular carcinoma; Spheroids; Drug resistance; Microcarrier; Epirubicin. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200015 Developed and validated stability indicating HPLC method for the determination of epirubicin in bulk drug, marketed injection and polymeric nanoparticles BJPS Tariq,Mohammad; Thomas,Shindu; Singh,Anu; Talegaonkar,Sushama. Present work is aimed to develop a simple, sensitive, robust and reliable HPLC method for routine quality control of epirubicin (EPI) in bulk drug, marketed injections and polymeric nanoparticles. Separation was carried out by C18 column. Isocratic elution was carried out using mobile phase A: 0.16% o-phosphoric acid solution, B: acetonitrile and methanol mixture (80:20, v/v) in the ratio of 60:40 (A: B) while the flow rate was maintained at 1mL/min. Analyses were performed at 233.5 nm using PDA detector. Excellent linear relationship was observed between peak-area versus drug concentration in the range of 1.0-100.0 μg/mL (r2, 0.999). Developed method was found to be sensitive (Limits of detection and quantification were found to be ~8 ng/mL and ~25 ng/mL,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Epirubicin; HPLC; Polymeric nanoparticles; Quality control; Forced degradation. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400619 Registros recuperados: 2 Primeira ... 1 ... Última

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