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The normal chain length distribution of the O antigen is required for the interaction of Shigella flexneri 2a with polarized Caco-2 cells Biol. Res.
Hoare,Anilei; Bravo,Denisse; Martinic,Mara; Valvano,Miguel A; Contreras,Inés; Álvarez,Sergio A.
Shigella flexneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. flexneri to adhere to and invade polarized Caco-2 cells. The S. flexneri 2a O antigen has two preferred chain lengths: a short O antigen (S-OAg) regulated by the WzzB protein and a very long O antigen (VL-OAg) regulated by Wzz pHS2. Mutants with defined deletions of the genes required for O-antigen assembly and polymerization were constructed and assayed for their abilities to adhere to and enter cultured epithelial cells. The results show that both VL- and S-OAg are required for invasion through the basolateral cell...
Tipo: Journal article Palavras-chave: Shigella; Epithelial cells; O antigen.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100003
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Cellular and matrix interactions during the development of T lymphocytes BJMBR
Owen,J.J.T.; McLoughlin,D.E.; Suniara,R.K.; Jenkinson,E.J..
The thymus contains an extensive extracellular matrix. Although thymocytes express integrins capable of binding to matrix molecules, the functional significance of the matrix for T cell development is uncertain. We have shown that the matrix is associated with thymic fibroblasts which are required for the CD44+ CD25+ stage of double negative (CD4-8-) thymocyte development. The survival of cells at this stage is dependent on IL-7 and we propose that the role of fibroblasts is to present, via the matrix, IL-7 to developing T cells.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular matrix; Epithelial cells; Fibroblasts; Integrins; Interleukin-7; Thymus.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500008
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Ultrastructure of the Secretory Epithelial Cells of the Bulbo-Urethral Glands (Cowper's gland) in the Indian Fruit Bat, Rousettus leschenaulti (Desmarest) During the Reproductive Cycle International Journal of Morphology
Nerkar,Archana A; Gujar,Maher P; Gadegone,Mohan M.
The present paper describes the ultrastructural characteristics of the bulbo-urethral gland (Cowper' glands) of the Indian fruit bat, R. leschenaulti during sexually inactive-breeding cycle. Cyclic histological changes during the seasonal breeding quiescence cycle are not well marked. There are no marked differences. The ultrastructural characteristic of the secretory epithelial cells of Bulbo-Uretrhal Gland gland have been studied during different phases of reproductive cycle. The secretory epithelial cells are characterized by the well-developed rough endoplasmic reticulum, extensive developed complexus golgiensis (Golgi apparatus) and mitochondria. Mitochondria with lamellate cristae are dispersed in the cytoplasm. Three different types of secretory...
Tipo: Journal article Palavras-chave: Chiroptera; Bat; Ultrastructure; Bulbo-urethral gland; Acini; Epithelial cells.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100035
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