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Evaluation of alveolar macrophage function after experimental infection with equine herpesvirus-1 in horses Arq. Bras. Med. Vet. Zootec.
Mori,E.; Mori,C.M.C.; Della Libera,A.M.M.P.; Lara,M.C.C.S.H.; Fernandes,W.R..
The role of the pulmonary alveolar macrophages (PAM) in the lung defense mechanism was evaluated in horses infected with equine hespesvirus-1 (EHV-1). Five adult horses were exposed to 10(6.6) TCID50 EHV-1 by intranasal instillation. Cytology of bronchoalveolar lavage (BAL) was performed using cytocentrifugation of samples and slides stained by Rosenfeld. Cell concentration was adjusted to 2<FONT FACE=Symbol>´</FONT>10(6) cells/ml, for the measurement of macrophage activity - spreading, phagocytosis of zymosan particles and release of hydrogen peroxide (H2O2). All animals were positive in virus isolation on the second, third and fifth days post-inoculation (DPI). Seroconversion was observed on the 14th DPI. Lymphocytosis was observed by BAL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine; Alveolar macrophage; Equine herpesvirus; EHV-1; Bronchoalveolar lavage.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300005
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Diversity of genomic electropherotypes of naturally occurring equine herpesvirus 1 isolates in Argentina BJMBR
Galosi,C.M.; Norimine,J.; Echeverría,M.G.; Oliva,G.A.; Nosetto,E.O.; Etcheverrigaray,M.E.; Tohya,Y.; Mikami,T..
The genomes of 10 equine herpesvirus 1 (EHV-1) strains isolated in Argentina from 1979 to 1991, and a Japanese HH1 reference strain were compared by restriction endonuclease analysis. Two restriction enzymes, BamHI and BglII, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. This suggests that the EHV-1 isolates studied, which circulated in Argentina for more than 10 years, belong to a single genotype.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Argentina; Electropherotypes; Equine herpesvirus.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600007
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