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| TAKABATAKE, Noriyuki; OKAMURA, Masashi; YOKOYAMA, Naoaki; OKUBO, Kazuhiro; IKEHARA, Yuzuru; IGARASHI, Ikuo; 横山, 直明; 五十嵐, 郁男. |
| Host sialic acid (SA) has recently been suggested to play an important role in erythrocyte (RBC) infection by Babesia spp. The present study attempted to further determine the specific type of SAs important in the RBC invasion. Bovine RBC was found to bear abundant α2-3-linked SA residues but not α2-6-linked SA in nature, confirmed by flow cytometric analysis of the neuraminidase (Nm)-treated RBCs. Lectin-blot analyses revealed the removal of α2-3-linked SAs from the 97-, 33-, and 31-kDa bands by the Nm treatment. Addition of the Nm-treated RBCs into an in vitro culture of B. bovis resulted in a decreased population of the parasitized RBCs. The thin smear samples from the cultures were then observed under a confocal laser scanning microscope after staining... |
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Palavras-chave: Babesia bovis; Erythrocyte; Invasion; Sialic acid. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4166 |
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| Carvalho, Elianora Gomes de; UFT; Seibert, Carla Simone; UFT; Coelho, Michella Soares; USP; Marques, Elineide Eugênio; UFT. |
| Neste estudo, foram avaliadas as características hematológicas e a morfometria de Auchenipterus nuchalis, Psectrogaster amazônica e Squaliforma emarginata, capturados no rio Tocantins. As amostragens foram realizadas no rio Tocantins, onde o sangue foi coletado por punção caudal dos animais, determinando-se hemograma completo e morfometria dos eritrócitos. Auchenipterus nuchalis (n = 15) apresentou maior número de eritrócitos, hemoglobina e hematócrito, seguido de P. amazônica (n = 26) e S. emarginata (n = 26). O tamanho dos eritrócitos foi maior para S. emarginata, mostrando que quanto maior o tamanho dos eritrócitos, menor o número de células circulantes. Squaliforma emarginata também apresentou maior número de blastos (eritrócitos ortocromáticos e... |
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Palavras-chave: 2.00.00.00-6 Ciências Biológicas peixes; Hematologia; Eritrócitos; Morfometria; Leucócitos; Rio Tocantins Fisiologia Comparada fish; Hematology; Erythrocyte; Morphometry; Leucocytes; Tocantins river. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1024 |
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| Cruz,Laura N.; Wu,Yang; Craig,Alister G.; Garcia,Célia R.S.. |
| Malaria is responsible for more than 1.5 million deaths each year, especially among children (Snow et al. 2005). Despite of the severity of malaria situation and great effort to the development of new drug targets (Yuan et al. 2011) there is still a relative low investment toward antimalarial drugs. Briefly there are targets classes of antimalarial drugs currently being tested including: kinases, proteases, ion channel of GPCR, nuclear receptor, among others (Gamo et al. 2010). Here we review malaria signal transduction pathways in Red Blood Cells (RBC) as well as infected RBCs and endothelial cells interactions, namely cytoadherence. The last process is thought to play an important role in the pathogenesis of severe malaria. The molecules displayed on the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytoadherence; Erythrocyte; Malaria; Plasmodium; Signal transduction. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200024 |
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| Mendanha,S.A.; Anjos,J.L.V.; Silva,A.H.M.; Alonso,A.. |
| Electron paramagnetic resonance (EPR) spectroscopy of spin labels was used to monitor membrane dynamic changes in erythrocytes subjected to oxidative stress with hydrogen peroxide (H2O2). The lipid spin label, 5-doxyl stearic acid, responded to dramatic reductions in membrane fluidity, which was correlated with increases in the protein content of the membrane. Membrane rigidity, associated with the binding of hemoglobin (Hb) to the erythrocyte membrane, was also indicated by a spin-labeled maleimide, 5-MSL, covalently bound to the sulfhydryl groups of membrane proteins. At 2% hematocrit, these alterations in membrane occurred at very low concentrations of H2O2 (50 µM) after only 5 min of incubation at 37°C in azide phosphate buffer, pH 7.4. Lipid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electron paramagnetic resonance; Spin label; Erythrocyte; Membrane fluidity; Membrane oxidation; Protein oxidation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600001 |
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| Ferreira,A.L.A.; Machado,P.E.A.; Matsubara,L.S.. |
| The free form of the iron ion is one of the strongest oxidizing agents in the cellular environment. The effect of iron at different concentrations (0, 1, 5, 10, 50, and 100 µM Fe3+) on the normal human red blood cell (RBC) antioxidant system was evaluated in vitro by measuring total (GSH) and oxidized (GSSG) glutathione levels, and superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and reductase (GSH-Rd) activities. Membrane lipid peroxidation was assessed by measuring thiobarbituric acid reactive substance (TBARS). The RBC were incubated with colloidal iron hydroxide and phosphate-buffered saline, pH 7.45, at 37oC, for 60 min. For each assay, the results for the control group were: a) GSH = 3.52 ± 0.27 µM/g Hb; b) GSSG = 0.17 ± 0.03... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Iron; Erythrocyte; Thiobarbituric acid reactive substance. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600004 |
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| Siqueira,A.J.S.; Remião,J.O.; Azevedo,A.M.P.; Azambuja,C.R.J.. |
| We describe a new gasometric method to determine erythrocyte catalase activity by the measurement of the volume of oxygen produced as a result of hydrogen peroxide decomposition in a system where enzyme and substrate are separated in a special reaction test tube connected to a manometer and the reagents are mixed with a motor-driven stirrer. The position of the reagents in the test tube permits the continuous measurement of oxygen evolution from the time of mixing, without the need to stop the reaction by the addition of acid after each incubation time. The enzyme activity is reported as KHb, i.e., mg hydrogen peroxide decomposed per second per gram of hemoglobin (s-1 g Hb-1). The value obtained for catalase activity in 28 samples of hemolyzed human blood... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Erythrocyte; Catalase assay; Gasometry. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900006 |
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