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| Lopez,Jose V; Ledger,Angela; Santiago-Vázquez,Lory Z; Pop,Mihai; Sommer,Dan D; Ranzer,Llanie K; Feldman,Robert A; Russell,G. Kerr. |
| Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non-“housekeeping” genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila... |
| Tipo: Journal article |
Palavras-chave: CDNA; Erythropodium caribaeorum; EST; Gorgonia ventalina; Gorgonian; Sea fan. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100008 |
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