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Irradiation effect on in vitro organogenesis, callus growth and plantlet development of Gerbera jamesonii Horticultura Brasileira
Hasbullah,Nor A; Taha,Rosna M; Saleh,Azani; Mahmad,Noraini.
The present work was carried out to study the effects of gamma irradiation on in vitro growth of explants, callus and the formation of shoots and plantlets. Irradiation is known to exhibit or inhibit the differentiation of cells and growth of plants in vitro, which helps in producing new plant varieties. Gamma irradiation is one of the physical mutagens that are widely used for mutation breeding. A gradual decline was observed in the number of shoots regenerated from irradiated petiole explants compared to control. Numbers of shoots regenerated from irradiated petiole explant cultured on Murashige & Skoog medium supplemented with 2.0 mg L-1 BAP and 0.5 mg L-1 NAA was reduced to 6.6±0.9 from 7.5±0.4 (control) when explants were exposed to 20 Gray of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gerbera jamesonii; Ornamental; Gamma irradiation; Explant; Callus regeneration; Plant growth regulator; MS medium.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000200012
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In vitro multiplication of 'Flordaguard' rootstock: cytokinin source and concentration effects, explants orientation and period of permanence in the culture medium BABT
Radmann,Elizete Beatriz; Bianchi,Valmor João; Fachinello,José Carlos; Ferreira,Letícia Vanni; Oliveira,Roberto Pedroso de.
This work aimed at studying the effects of different sources and cytokinin concentrations, as well as explants orientation and time of their permanence in shoots induction medium to obtain high multiplication rate. Four experiments were carried out to evaluate the sprout percentage mean, shoots number per explant, elongated shoots percentage and shoots length. The treatments with 2iP and zeatin did not promote in vitro plant multiplication. When different BAP concentrations were tested, an increase in sprout percentage was obtained using BAP concentration up to 3.66 mg L-1 (80%), and for shoots number per explant a linear behavior was observed, showing a mean of four shoots per explant with 4.0 mg L-1 BAP. For the 'Flordaguard' rootstocks, the explants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Cytokinin; Explant; Prunus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100004
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