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An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis Genet. Mol. Biol.
Lucheta,Adriano R.; Silva-Pinhati,Ana Carla O.; Basílio-Palmieri,Ana Carolina; Berger,Irving J.; Freitas-Astúa,Juliana; Cristofani,Mariângela.
Citrus species are known by their high content of phenolic compounds, including a wide range of flavonoids. In plants, these compounds are involved in protection against biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. In humans, flavonoid consumption has been related to increasing overall health and fighting some important diseases. The goals of this study were to identify expressed sequence tags (EST) in Citrus sinensis (L.) Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids). A thorough analysis of all related putative genes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenylpropanoids; Sweet orange; Expressed sequence tags (EST); Secondary metabolism.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500010
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Development and validation of new SSR markers from expressed regions in the garlic genome Scientia Agricola
Ipek,Meryem; Sahin,Nihan; Ipek,Ahmet; Cansev,Asuman; Simon,Philipp W..
Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs) at the publicly available GarlicEST database were screened for SSR motifs and a total of 132 SSR motifs were identified. Primer pairs were designed for 50 SSR motifs and 24 of these primer pairs were selected as SSR markers based on their consistent amplification patterns and polymorphisms. In addition, two SSR markers were developed from the sequences of garlic cDNA-AFLP fragments. The use of 26 EST-SSR markers for the assessment of genetic relationship was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags (EST); Simple sequence repeat markers; Genetic relationship.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100041
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