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| Sawaya,Alexandra C. H. F.; Souza,Kirllian S.; Marcucci,Maria C.; Cunha,Ildenize B.S.; Shimizu,Mario T.. |
| Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propolis; Thin layer chromatography; Bioauthography; High performance liquid chromatography; Extraction methods; Minimal bactericidal concentration. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100017 |
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| Montiel-Montoya,Jorge; Valdez-Morales,Maribel; Reyes,Cesar; Barrales-Cureño,Hebert Jair. |
| ABSTRACT: Two chemical treatments, five enzymatic (pectinase, lipase, hemicellulase, hemicellulose-cellulase or lipase-pectinase) and one microbiological (Bacillus subtilis) treatment were evaluated to obtain glucosamine hydrochloride (Gluc-HCl) from the chitin obtained from crab (Callinectes bellicosus) exoskeletons. Chemical treatments were referred as Method A (HCl hydrolysis during 75 min at 90°C) and Method B (HCl hydrolysis during 20 min and 14 h of rest). Glucosamine and, in some cases, N-acetyl-D-glucosamine were identified and quantified by HPLC. Treatments with the greater concentrations of Gluc-HCl in descending order were: lipase (94.4 mg/g), microbiological (45.7 mg/g), lipase-pectinase (22.9 mg/g), hemicellulase-cellulase (20.9 mg/g),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Callinectes bellicosus; Extraction methods; Glucosamine hydrochloride; HPLC analysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900752 |
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