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| Pereira,Marlon Corrêa; Vieira,Nívea Moreira; Tótola,Marcos Rogério; Kasuya,Maria Catarina Megumi. |
| Rhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characterization and analysis of conserved sequences of genomic DNA are frequently employed in the identification and study of fungi diversity. However, phytopathogenic Rhizoctonia-like fungi have been reliably and accurately characterized and identified through the examination of the fatty acid composition. To evaluate the efficacy of fatty acid composition in characterizing and identifying Rhizoctonia-like mycorrhizal fungi in orchids, three Epulorhiza spp. mycorrhizal fungi from Epidendrum secundum, two unidentified fungi isolated from Epidendrum denticulatum, and a phytopathogenic fungus, Ceratorhiza sp. AGC, were grouped based on the profile of their fatty acids,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizoctonia-like fungi; Epidendrum spp. orchids; FAME; MIDI method; ITS sequence analysis; Fungos rizoctonioides; Orquídeas Epidendrum spp; FAME; Método MIDI; Análise da sequência da região ITS. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000400009 |
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| Parvathi,Ammini; Krishna,Kiran; Jose,Jiya; Joseph,Neetha; Nair,Santha. |
| Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; FAME; 16S rDNA; CesB; AP-PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200012 |
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