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Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil BJM
Henzel,Andréia; Brum,Mário Celso Sperotto; Lautert,Cláudia; Martins,Mathias; Lovato,Luciane Teresinha; Weiblen,Rudi.
Feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1) are the two primary causes of upper respiratory tract disease in cats. The aim of this study was to demonstrate the distribution of FCV and FHV-1 among the feline population of several counties in Rio Grande do Sul State, Brazil. To this end, conjunctival and nasal swabs were collected from 302 cats from different locations, including households, breeding catteries, veterinary clinics, animal hospitals and experimental research facilities. The samples were collected between July 2006 to June 2009. The virus isolation was performed in CRFK cells and, subsequently, the identification was confirmed by PCR. FCV, FHV-1, or both were isolated from 55 cats from 28 different locations. FCV alone was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FCV; FHV-1; URTD; Epidemiology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200017
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Feline herpesvirus 1 viral load related to environmental factors in sheltered cats Ciência Rural
Baumworcel,Natasha; Pereira,Joylson de Jesus; Soares,Ana Maria Barros; Souza,Guilherme Nunes; Almosny,Nadia Regina Pereira; Castro,Tatiana Xavier de.
ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection....
Tipo: Info:eu-repo/semantics/article Palavras-chave: FHV-1; Conjunctivitis; Shelter medicine; QPCR; Animal shelter.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454
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