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GARCÍA,KATHERINE P; RUBILAR,PAULINA S; VARGAS,MACARENA F; CÁRDENAS,HUGO; RIOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H ; FUHRER,JUAN; HECKELS,JOHN E; CHRISTODOULIDES,MYRON; VELÁSQUEZ,LUIS A. |
In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS. Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release,... |
Tipo: Journal article |
Palavras-chave: Cellular damage; Fallopian tubes; Neisseria gonorrhoeae; Nitric oxide. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100006 |
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Costoya,Alberto; Morales,Francisco; Borda,Paula; Vargas,Renato; Fuhrer,Juan; Salgado,Nicole; Cárdenas,Hugo; Velasquez,Luis. |
BACKGROUND: The role of mycoplasmas on the development and sequelae of pelvic inflammatory disease remains controversial. The objective of the present study is to correlate directly the presence of Mycoplasmateceae through polimerase chain reaction (PCR) determinations in cervix and Fallopian tubes of infertile patients with tubo-peritoneal factor diagnosed through laparoscopy. METHODS: Thirty patients with tubo-peritoneal infertility and 30 normal fertile patients were included in the study; cervical samples and tubal flushings were obtained during laparoscopy. PCR determinations for the detection of genetic material of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealiticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Mycoplasmateceae; Fallopian tubes; Tuboperitoneal infertility; Infections transmitted sexually. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300010 |
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