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Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci Genet. Mol. Biol.
Mantellatto,Aline Meira Bonfim; Caparroz,Renato; Christofoletti,Maurício Durante; Piovezan,Ubiratan; Duarte,José Maurício Barbanti.
Abstract The pampas deer (Ozotoceros bezoarticus) is close to being classified as ‘globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Fecal DNA; Microsatellites; Non-invasive methods; Pampas deer.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500774
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Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction Genet. Mol. Biol.
Chaves,Paulo B.; Paes,Marcela F.; Mendes,Sérgio L.; Strier,Karen B.; Louro,Iúri D.; Fagundes,Valéria.
The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachyteles; Conservation genetics; Endangered species; Fecal DNA; Noninvasive sampling.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400028
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Genetic structure in two northern muriqui populations (Brachyteles hypoxanthus, Primates, Atelidae) as inferred from fecal DNA Genet. Mol. Biol.
Fagundes,Valéria; Paes,Marcela F.; Chaves,Paulo B.; Mendes,Sérgio L.; Possamai,Carla de B.; Boubli,Jean P.; Strier,Karen B..
We assessed the genetic diversity of two northern muriqui (Brachyteles hypoxanthus Primata, Atelidae) populations, the Feliciano Miguel Abdala population (FMA, n = 108) in the Brazilian state of Minas Gerais (19°44' S, 41°49' W) and the Santa Maria de Jetibá population (SMJ, n = 18) in the Brazilian state of Espírito Santo (20°01' S, 40°44' W). Fecal DNA was isolated and PCR-RFLP analysis used to analyze 2160 bp of mitochondrial DNA, made up of an 820 bp segment of the gene cytochrome c oxidase subunit 2 (cox2, EC 1.9.3.1), an 880 bp segment of the gene cytochrome b (cytb, EC 1.10.2.2) and 460 bp of the hypervariable segment of the mtDNA control region (HVRI). The cox2 and cytb sequences were monomorphic within and between populations whereas the HVRI...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Brachyteles; Conservation genetics; Fecal DNA; MtDNA; PCR-RFLP.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100028
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Non invasive methods for genetic analysis applied to ecological and behavioral studies in Latino-America R. Bras. Zootec.
González,Susana; Duarte,José Mauricio Barbanti.
Documenting the presence and abundance of the neotropical mammals is the first step for understanding their population ecology, behavior and genetic dynamics in designing conservation plans. The combination of field research with molecular genetics techniques are new tools that provide valuable biological information avoiding the disturbance in the ecosystems, trying to minimize the human impact in the process to gather biological information. The objective of this paper is to review the available non invasive sampling techniques that have been used in Neotropical mammal studies to apply to determine the presence and abundance, population structure, sex ratio, taxonomic diagnostic using mitochondrial markers, and assessing genetic variability using nuclear...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptic species; Fecal DNA; Non invasive sampling.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007001000011
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Re-discovering jaguar in remaining coastal Atlantic Forest in southeastern Brazil by non-invasive DNA analysis Biota Neotropica
Souza,Andiara Silos Moraes de Castro; Saranholi,Bruno Henrique; Crawshaw Jr.,Peter Gransden; Paviolo,Agustin Javier; Rampim,Lilian Elaine; Sartorello,Leonardo; Galetti Jr.,Pedro Manoel.
Abstract Jaguar populations have been declining in Brazil mostly due to habitat loss and fragmentation, conflict with humans, poaching and reduction of prey. This is dramatically true in the Atlantic Forest, where occurrence of this large felid is now restricted to very few remaining areas. We used a non-invasive DNA analysis to search through felid scats collected in the Santa Virginia Unit (SVU) of the Serra do Mar State Park, to test for the potential presence of jaguar there. Our results indicated at least three individuals (two females and one male) inside SVU, thus confirming at least temporary presence of this top predator in this important protected area. It is now crucial to intensify studies in that area and surroundings, to evaluate the status...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Panthera onca; Fecal DNA; Santa Virginia Unit; Serra do Mar State Park.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032017000200501
Registros recuperados: 5
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